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glpF glycerol facilitator [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948422, updated on 3-Dec-2024

Summary

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Official Symbol
glpF
Official Full Name
glycerol facilitator
Primary source
ECOCYC:EG10396
Locus tag
b3927
See related
ASAP:ABE-0012828
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3919
Summary
The glycerol facilitator, GlpF, allows the facilitated diffusion of glycerol across the inner membrane for utilization inside the cell. [More information is available at EcoCyc: EG10396].
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Genomic context

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Sequence:
NC_000913.3 (4117245..4118090, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene fructose-1,6-bisphosphatase 2 Neighboring gene glycerol kinase Neighboring gene cell division factor ZapB Neighboring gene ribonuclease E inhibitor protein A

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Preparation of glycerol facilitator for protein structure and folding studies in solution. Manley D, et al. Methods Mol Biol, 2003. PMID 12824546
  2. Bioinformatics. Reality simulation--observe while it happens. Berendsen HJ. Science, 2001 Dec 14. PMID 11743188
  3. Role of C-terminal domain and transmembrane helices 5 and 6 in function and quaternary structure of major intrinsic proteins: analysis of aquaporin/glycerol facilitator chimeric proteins. Duchesne L, et al. J Biol Chem, 2002 Jun 7. PMID 11927589
  4. Water transporters: how so fast yet so selective? Law RJ, et al. Curr Biol, 2002 Apr 2. PMID 11937042
  5. Aquaglyceroporins: channel proteins with a conserved core, multiple functions, and variable surfaces. Engel A, et al. Int Rev Cytol, 2002. PMID 11952238

See all (98) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Multiple pore lining residues modulate water permeability of GlpF.
    Title: Multiple pore lining residues modulate water permeability of GlpF.
  2. Mutation of tryptophan Trp219 affects the aqualgyeroporin GlpF activity and the stability of the active GlpF tetramer.
    Title: The GlpF residue Trp219 is part of an amino-acid cluster crucial for aquaglyceroporin oligomerization and function.
  3. Osmotic water permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations have been presented.
    Title: Computing osmotic permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations.
  4. Folding of the aquaglyceroporin GlpF determines its stability. (Review)
    Title: Folding and stability of the aquaglyceroporin GlpF: Implications for human aqua(glycero)porin diseases.
  5. TMAO increases the heat stability of the GF tetramer an average of 10 degrees C in the 4 detergents and also protects the protein from denaturation by SDS.
    Title: The effects of a protein osmolyte on the stability of the integral membrane protein glycerol facilitator.
  6. The tetrameric alpha-helical GlpF unfolds via a dimeric folding intermediate.
    Title: The tetrameric α-helical membrane protein GlpF unfolds via a dimeric folding intermediate.
  7. GlpF exists as a stably folded tetramer in detergent solutions of neutral dodecyl maltoside and in zwitterionic lysomyristoylphosphatidylcholine.
    Title: Stability of the glycerol facilitator in detergent solutions.
  8. GlpF is found to be highly permeable for small solutes and permeable for larger solutes.
    Title: Mechanism of selectivity in aquaporins and aquaglyceroporins.
  9. analysis of of Escherichia coli aquaporin AqpZ and GlpF single-channel water permeabilities
    Title: Single-channel water permeabilities of Escherichia coli aquaporins AqpZ and GlpF.
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General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables channel activity IEA
Inferred from Electronic Annotation
more info
  
enables glycerol channel activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glycerol channel activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glycerol transmembrane transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
NOT enables water transmembrane transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in cellular response to mercury ion IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glycerol transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glycerol transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glycerol transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glycerol transmembrane transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
NOT involved_in water transport IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
glycerol facilitator

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4117245..4118090 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418362.1 glycerol facilitator [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418362.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6C9Q6C8, A0A7D7PKY3
    Conserved Domains (1) summary
    cd00333
    Location:10 → 254
    MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...
Nucleotide Protein
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