NCR1 sphingolipid transporter [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NCR1
Official Full Name: sphingolipid transporter
Primary source: SGD:S000005927
Locus tag: YPL006W
See related: AllianceGenome:SGD:S000005927; FungiDB:YPL006W; VEuPathDB:YPL006W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables sterol binding activity. Involved in sphingolipid metabolic process and sterol transport. Located in fungal-type vacuole membrane. Used to study Niemann-Pick disease and Niemann-Pick disease type C1. Human ortholog(s) of this gene implicated in Niemann-Pick disease and Niemann-Pick disease type C1. Orthologous to several human genes including NPC1 (NPC intracellular cholesterol transporter 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See NCR1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (544631..548143)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural and biochemical analysis of ligand binding in yeast Niemann-Pick type C1-related protein.
Title: Structural and biochemical analysis of ligand binding in yeast Niemann-Pick type C1-related protein.
this study presents four structures of yeast NCR1 and NPC2.
Title: Structural Insight into Eukaryotic Sterol Transport through Niemann-Pick Type C Proteins.
These data show that Ncr1p and Npc2p proteins promote microautophagy in stationary phase and under nitrogen starvation conditions, likely by increasing sterol in the limiting membrane of the vacuole.
Title: Niemann-Pick type C proteins promote microautophagy by expanding raft-like membrane domains in the yeast vacuole.
Authors show that yeast cells lacking Ncr1p, an orthologue of mammalian NPC1, exhibited a higher sensitivity to hydrogen peroxide and a shortened chronological lifespan.
Title: Sphingolipid signalling mediates mitochondrial dysfunctions and reduced chronological lifespan in the yeast model of Niemann-Pick type C1.
Results identify the first phenotype caused by deletion of NCR1 from the yeast genome, resistance to the ether lipid drug, edelfosine.
Title: A yeast model system for functional analysis of the Niemann-Pick type C protein 1 homolog, Ncr1p.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables sterol binding	IBA Inferred from Biological aspect of Ancestor more info
enables sterol binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in lipid transport	IEA Inferred from Electronic Annotation more info
involved_in sphingolipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in sphingolipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sterol transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in sterol transport	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
located_in fungal-type vacuole membrane	HDA more info	PubMed
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: sphingolipid transporter

NP_015319.1

Vacuolar membrane protein involved in sterol trafficking with Npc2p; conformational changes drive sterol translocation across the glycocalyx and into the vacuolar membrane; involved in sphingolipid metabolism; cells lacking Ncr1p exhibit high levels of long chain bases (LCBs), similar to accumulation of lipids observed in patients with Neimann-Pick C disease due to loss-of-function mutations in human NPC1; transits through the vacuolar protein sorting pathway via the AP-3 pathway

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

544631..548143

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183820.1 → NP_015319.1 TPA: sphingolipid transporter [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015319.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W406, Q12200

UniProtKB/TrEMBL

B3LL56, C8ZJ20, N1NVZ9

Conserved Domains (1) summary

TIGR00917
Location:23 → 1140

2A060601; Niemann-Pick C type protein family