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Envelope surface glycoprotein gp120
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env
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Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells |
PubMed
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Gag-Pol
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gag-pol
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Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells |
PubMed
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Nef
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nef
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Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells |
PubMed
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Pr55(Gag)
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gag
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HIV-1 Gag interacts with RPS6 as demonstrated by proximity dependent biotinylation proteomics |
PubMed
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gag
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Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells |
PubMed
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Rev
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rev
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DDX5 enhances the Rev-dependent HIV-1 RNA export and the interaction between DDX5 and Rev is largely dependent on RNA |
PubMed
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rev
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DDX5 interacts with HIV-1 Rev and enhances the Rev function. DDX5 co-localizes with Rev in both nucleolus and nucleus |
PubMed
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rev
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DDX5 interacts with DDX1, DDX3, or DDX21 and synergistically enhances the Rev function |
PubMed
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rev
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HIV-1 Rev interacting protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with DDX5 is increased by RRE |
PubMed
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Vpr
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vpr
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A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies upregulation of DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5) expression by HIV-1 Vpr in Vpr transduced macrophages |
PubMed
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capsid
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gag
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siRNA knockdown of DDX5 decreases intra- and extra-cellular HIV CA(p24) from HeLa cells transfected with env-deleted HIV-1 plasmid, a vesicular stomatitis virus glycoprotein plasmid and specific siRNA. Resulting HIV-1 demonstrates decreased infectivity. |
PubMed
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gag
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Knockdown of DDX5 by siRNA inhibits HIV-1 replication with downregulated levels of HIV-1 CA expression in CD4+ T cells |
PubMed
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matrix
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gag
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HIV-1 MA upregulates DDX5 gene expression in HepG2 cells |
PubMed
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retropepsin
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gag-pol
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Positional proteomics analysis identifies the cleavage of human DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5) at amino acid residues 96-97 by the HIV-1 protease |
PubMed
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