zen-4 Kinesin-like protein [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: zen-4
Official Full Name: Kinesin-like protein
Primary source: WormBase:WBGene00006974
Locus tag: CELE_M03D4.1
See related: AllianceGenome:WB:WBGene00006974
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables several functions, including identical protein binding activity; microtubule binding activity; and plus-end-directed microtubule motor activity. Involved in several processes, including nuclear division; pronuclear migration; and regulation of actomyosin contractile ring contraction. Located in cleavage furrow; microtubule cytoskeleton; and midbody. Part of centralspindlin complex. Is expressed in germ cell and gonad. Human ortholog(s) of this gene implicated in congenital dyserythropoietic anemia type III. Orthologous to human KIF23 (kinesin family member 23). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See zen-4 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 9

Sequence:: Chromosome: IV; NC_003282.8 (6118215..6121777)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study shows that oocyte production by the syncytial Caenorhabditis elegans germline requires CYK-4 but not ZEN-4, which contrasts with cytokinesis, where both are essential.
Title: CYK-4 functions independently of its centralspindlin partner ZEN-4 to cellularize oocytes in germline syncytia.
the crystal structure of kinesin-6 Zen4 in a nucleotide-free, apo state, with the NL initial segment (NIS) adopting a backward-docked conformation and the preceding alpha6 helix partially melted, is reported.
Title: Crystal structure of Zen4 in the apo state reveals a missing conformation of kinesin.
Centralspindlin is a 2:2 heterotetramer of ZEN-4 and CYK4.
Title: Direct interaction between centralspindlin and PRC1 reinforces mechanical resilience of the central spindle.
CYK-4 binding decreases the rate of ZEN-4-mediated microtubule gliding. These results constrain models for the molecular organization of centralspindlin.
Title: Binding of the CYK-4 subunit of the centralspindlin complex induces a large scale conformational change in the kinesin subunit.
Centralspindlin function at the rachis bridge involves ZEN-4 action on the microtubules.
Title: A postmitotic function and distinct localization mechanism for centralspindlin at a stable intercellular bridge.
results indicate zygotic zen-4 function is required for correct division of epidermal precursors and, hence, indirectly for normal morphogenesis and that the epidermal morphogenetic program is surprisingly robust even in the absence of zen-4 function
Title: Zygotic loss of ZEN-4/MKLP1 results in disruption of epidermal morphogenesis in the C. elegans embryo.
The structural features of the interactions between monomeric and dimeric motor domain constructs of ZEN-4 and microtubules.
Title: Structural analysis of the ZEN-4/CeMKLP1 motor domain and its interaction with microtubules.
ZEN-4/MKLP1 and CYK-4/RhoGAP regulate an early step in epithelial polarization that is required to establish the apical domain and apical junctions .
Title: ZEN-4/MKLP1 is required to polarize the foregut epithelium.
phosphoregulation of the motor domain of Zen-4 kinesin ensures that central spindle assembly occurs at the appropriate time in the cell cycle and maintains genomic stability
Title: Cell cycle regulation of central spindle assembly.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

M03D4.1 (e-PCR)
- UniSTS:317514

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables cytoskeletal motor activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables microtubule motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed
enables microtubule motor activity	IEA Inferred from Electronic Annotation more info
enables plus-end-directed microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed
enables plus-end-directed microtubule motor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in meiotic spindle midzone assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule-based movement	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule-based movement	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule-based movement	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid segregation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic spindle midzone assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle midzone assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polar body extrusion after meiotic divisions	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pronuclear migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of actomyosin contractile ring contraction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of actomyosin contractile ring contraction	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of centralspindlin complex	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in cleavage furrow	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
part_of kinesin complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in midbody	IDA Inferred from Direct Assay more info	PubMed
located_in mitotic spindle midzone	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle midzone	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Kinesin-like protein

NP_001023309.1

Confirmed by transcript evidence

NP_001023310.1

Confirmed by transcript evidence

NP_001023311.1

Confirmed by transcript evidence

NP_001360082.1

Confirmed by transcript evidence

NP_001360295.1

Confirmed by transcript evidence

NP_001360296.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

6118215..6121777

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001372836.1 → NP_001360082.1 Kinesin-like protein [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

Q8MQ03

Conserved Domains (2) summary

pfam16540
Location:674 → 748

MKLP1_Arf_bdg; Arf6-interacting domain of mitotic kinesin-like protein 1

cl22853
Location:26 → 426

Motor_domain; Myosin and Kinesin motor domain
NM_001372837.3 → NP_001360296.1 Kinesin-like protein [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

V6CL80

Conserved Domains (2) summary

pfam16540
Location:672 → 746

MKLP1_Arf_bdg; Arf6-interacting domain of mitotic kinesin-like protein 1

cl22853
Location:26 → 424

Motor_domain; Myosin and Kinesin motor domain
NM_001028140.5 → NP_001023311.1 Kinesin-like protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023311.1

Status: REVIEWED

UniProtKB/TrEMBL

Q8MQ04

Conserved Domains (3) summary

smart00129
Location:27 → 434

KISc; Kinesin motor, catalytic domain. ATPase

cl22853
Location:26 → 426

Motor_domain; Myosin and Kinesin motor domain

pfam16540
Location:674 → 773

MKLP1_Arf_bdg; Arf6-interacting domain of mitotic kinesin-like protein 1
NM_001028138.3 → NP_001023309.1 Kinesin-like protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023309.1

Status: REVIEWED

UniProtKB/TrEMBL

G5EG83

Conserved Domains (3) summary

smart00129
Location:27 → 432

KISc; Kinesin motor, catalytic domain. ATPase

cl22853
Location:26 → 424

Motor_domain; Myosin and Kinesin motor domain

pfam16540
Location:672 → 771

MKLP1_Arf_bdg; Arf6-interacting domain of mitotic kinesin-like protein 1
NM_001372838.2 → NP_001360295.1 Kinesin-like protein [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

V6CLB1

Conserved Domains (2) summary

pfam16540
Location:674 → 747

MKLP1_Arf_bdg; Arf6-interacting domain of mitotic kinesin-like protein 1

cl22853
Location:26 → 426

Motor_domain; Myosin and Kinesin motor domain
NM_001028139.3 → NP_001023310.1 Kinesin-like protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023310.1

Status: REVIEWED

UniProtKB/TrEMBL

G5EC43

Conserved Domains (3) summary

smart00129
Location:27 → 432

KISc; Kinesin motor, catalytic domain. ATPase

cl22853
Location:26 → 424

Motor_domain; Myosin and Kinesin motor domain

pfam16540
Location:672 → 745

MKLP1_Arf_bdg; Arf6-interacting domain of mitotic kinesin-like protein 1