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    tolA Tol-Pal system protein TolA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946625, updated on 3-Dec-2024

    Summary

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    Official Symbol
    tolA
    Official Full Name
    Tol-Pal system protein TolA
    Primary source
    ECOCYC:EG11007
    Locus tag
    b0739
    See related
    ASAP:ABE-0002515
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    cim; ECK0728; excC; lky; tol-2
    Summary
    Cell envelope integrity is stabilized by the Tol-Pal system linking inner and outer membranes. [More information is available at EcoGene: EG11007]. TolA is one of three (along with TolQ and TolR) inner membrane protein members of the YbgC-YbgF-TolQ-R-A-B-Pal Cell Envelope Complex, also known as the Tol-Pal system. [More information is available at EcoCyc: EG11007].
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    Genomic context

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    Sequence:
    NC_000913.3 (776342..777607)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene esterase/thioesterase Neighboring gene Tol-Pal system protein TolQ Neighboring gene Tol-Pal system protein TolR Neighboring gene Tol-Pal system periplasmic protein TolB Neighboring gene peptidoglycan-associated outer membrane lipoprotein Pal

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Differential complementation of DeltatolA Escherichia coli by a Yersinia enterocolitica TolA homologue. Weitzel AC, et al. FEMS Microbiol Lett, 2008 May. PMID 18355278
    2. Discovery of critical Tol A-binding residues in the bactericidal toxin colicin N: a biophysical approach. Raggett EM, et al. Mol Microbiol, 1998 Jun. PMID 9680221
    3. Solution structure of the E.coli TolA C-terminal domain reveals conformational changes upon binding to the phage g3p N-terminal domain. Deprez C, et al. J Mol Biol, 2005 Mar 4. PMID 15701516
    4. The TolA protein interacts with colicin E1 differently than with other group A colicins. Schendel SL, et al. J Bacteriol, 1997 Jun. PMID 9171417, Free PMC Article
    5. TolA central domain interacts with Escherichia coli porins. Derouiche R, et al. EMBO J, 1996 Dec 2. PMID 8978668, Free PMC Article

    See all (75) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Colicin N and M13 phage protein G3p compete with TolB for a binding site on TolA.
      Title: Antibacterial toxin colicin N and phage protein G3p compete with TolB for a binding site on TolA.
    2. Despite elevated virulence gene transcription, in tolA deleted strains virulence of enterohemorrhagic Escherichia coli in the Galleria mellonella wax worm model was substantially attenuated.
      Title: The role for TolA in enterohemorrhagic Escherichia coli pathogenesis and virulence gene transcription.
    3. Variation of intragenic tandem repeat tract of tolA modulates Escherichia coli stress tolerance.
      Title: Variation of intragenic tandem repeat tract of tolA modulates Escherichia coli stress tolerance.
    4. Using a combination of in vivo coimmunoprecipitations and in vitro pulldown experiments, we demonstrated that KT interacts with the TolA, TolB, and TolR proteins.
      Title: Interaction of the colicin K bactericidal toxin with components of its import machinery in the periplasm of Escherichia coli.
    5. The authors show that the stable YbgF trimer undergoes a marked change in oligomeric state on binding TolA, forming a one-to-one complex with the Tol protein.
      Title: TolA modulates the oligomeric status of YbgF in the bacterial periplasm.
    6. energy transduced from the cytoplasmic membrane to the outer membrane by TolA could be the driving force for immunity protein release and concomitant translocation of the nuclease domain
      Title: Energy-dependent immunity protein release during tol-dependent nuclease colicin translocation.
    7. The Y. enterocolitica TolA supported the physiological function of the Tol system in E. coli, and conferred sensitivity to the TolA-dependent colicins.
      Title: Differential complementation of DeltatolA Escherichia coli by a Yersinia enterocolitica TolA homologue.
    8. 3D structure of free TolAIII by heteronuclear NMR spectroscopy
      Title: Solution structure of the E.coli TolA C-terminal domain reveals conformational changes upon binding to the phage g3p N-terminal domain.
    9. in vivo and in vitro coprecipitation analyses demonstrated that colicin A and g3p N-terminal domains compete for binding to TolA.
      Title: Tol-dependent macromolecule import through the Escherichia coli cell envelope requires the presence of an exposed TolA binding motif.
    10. absence of TolA and Pal elicits a sustained extracytoplasmic stress response that in turn reduces O-antigen polymerization but does not affect the stability of the Wzy O-antigen polymerase
      Title: Defective O-antigen polymerization in tolA and pal mutants of Escherichia coli in response to extracytoplasmic stress.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables disordered domain specific binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein domain specific binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables toxin transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables virion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in bacteriocin transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within bacteriocin transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within cell division IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cell division IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cell division NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within cell septum assembly EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within cellular response to bacteriocin IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein import IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of membrane invagination NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within symbiont entry into host cell IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within symbiont entry into host cell IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cell division site IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Tol-Pal system protein TolA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      776342..777607
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415267.1 Tol-Pal system protein TolA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415267.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8I0CBZ0
      Conserved Domains (1) summary
      PRK09510
      Location:1 → 421
      tolA; cell envelope integrity inner membrane protein TolA; Provisional
    Nucleotide Protein
    Heading Accession and Version

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