U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    csgB curlin, minor subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947391, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    csgB
    Official Full Name
    curlin, minor subunit
    Primary source
    ECOCYC:G6547
    Locus tag
    b1041
    See related
    ASAP:ABE-0003534
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1027
    Summary
    The csgAB operon is regulated by CsgD. [More information is available at EcoGene: EG12621]. CsgB is the minor curlin subunit, which appears to function as a nucleator in the assembly of curli on the cell surface . [More information is available at EcoCyc: G6547].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (1103951..1104406)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene curli assembly component CsgE Neighboring gene DNA-binding transcriptional dual regulator CsgD Neighboring gene curlin, major subunit Neighboring gene curlin chaperone

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Nucleator function of CsgB for the assembly of adhesive surface organelles in Escherichia coli. Bian Z, et al. EMBO J, 1997 Oct 1. PMID 9312041, Free PMC Article
    2. Curli provide the template for understanding controlled amyloid propagation. Wang X, et al. Prion, 2008 Apr-Jun. PMID 19098444, Free PMC Article
    3. Curli fibers are required for development of biofilm architecture in Escherichia coli K-12 and enhance bacterial adherence to human uroepithelial cells. Kikuchi T, et al. Microbiol Immunol, 2005. PMID 16172544
    4. The curli nucleator protein, CsgB, contains an amyloidogenic domain that directs CsgA polymerization. Hammer ND, et al. Proc Natl Acad Sci U S A, 2007 Jul 24. PMID 17636121, Free PMC Article
    5. The molecular basis of functional bacterial amyloid polymerization and nucleation. Wang X, et al. J Biol Chem, 2008 Aug 1. PMID 18508760, Free PMC Article

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Inactivation of the cysH gene results in increased surface adhesion and cell aggregation by activating the expression of the curli-encoding csgBAC operon via phosphoadenosine 59-phosphosulfate accumulation.
      Title: Sulfate assimilation pathway intermediate phosphoadenosine 59-phosphosulfate acts as a signal molecule affecting production of curli fibres in Escherichia coli.
    2. Constructed DeltatolC mutant and complement extraintestinal pathogenic E. coli (ExPEC) strains to study the role of TolC in the retention of biofilm formation and curli production capability under different osmotic conditions.
      Title: TolC promotes ExPEC biofilm formation and curli production in response to medium osmolarity.
    3. Substoichiometric concentrations of CsgB induce a change in the mechanism of CsgA aggregation from that of forming amorphous aggregates to that of structured intermediates similar to those of CsgB alone
      Title: The E. coli CsgB nucleator of curli assembles to β-sheet oligomers that alter the CsgA fibrillization mechanism.
    4. fibrils formed by CsgA and CsgB, the primary curli proteins of Escherichia coli, possess many of the hallmarks typical of amyloid
      Title: The functional curli amyloid is not based on in-register parallel beta-sheet structure.
    5. CsgD overexpression can overcome temperature-dependent control of the curli-encoding csgBA operon, but not of the cellulose-related adrA gene
      Title: Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Escherichia coli.
    6. CsgB(trunc) was only able to act as a nucleator when cells were genetically manipulated to secrete higher concentrations of CsgA
      Title: The curli nucleator protein, CsgB, contains an amyloidogenic domain that directs CsgA polymerization.
    Submit: New GeneRIF Correction

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within amyloid fibril formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in amyloid fibril formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cell adhesion IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within curli assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of amyloid fibril formation EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in regulation of amyloid fibril formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within single-species biofilm formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in pilus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in pilus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    curlin, minor subunit

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1103951..1104406
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415559.1 curlin, minor subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415559.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P0ABK7
      Conserved Domains (1) summary
      PRK10101
      Location:1 → 151
      csgB; curlin minor subunit CsgB; Provisional
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.