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    holE DNA polymerase III subunit theta [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947471, updated on 17-Dec-2024

    Summary

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    Official Symbol
    holE
    Official Full Name
    DNA polymerase III subunit theta
    Primary source
    ECOCYC:EG11505
    Locus tag
    b1842
    See related
    ASAP:ABE-0006126
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1843
    Summary
    The theta subunit of DNA polymerase III binds tightly to the epsilon subunit but not to the alpha subunit . [More information is available at EcoCyc: EG11505].
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    Genomic context

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    Sequence:
    NC_000913.3 (1925108..1925338)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene CopD family protein Neighboring gene copper-binding protein Neighboring gene putative carbon-nitrogen hydrolase family protein YobB Neighboring gene exonuclease X Neighboring gene oligopeptidase B

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Phage like it HOT: solution structure of the bacteriophage P1-encoded HOT protein, a homolog of the theta subunit of E. coli DNA polymerase III. Derose EF, et al. Structure, 2004 Dec. PMID 15576035
    2. A preliminary CD and NMR study of the Escherichia coli DNA polymerase III theta subunit. Li D, et al. Proteins, 1999 Jul 1. PMID 10373010
    3. The bacteriophage P1 hot gene, encoding a homolog of the E. coli DNA polymerase III theta subunit, is expressed during both lysogenic and lytic growth stages. Chikova AK, et al. Mutat Res, 2007 Nov 1. PMID 17482649, Free PMC Article
    4. Nuclear magnetic resonance solution structure of the Escherichia coli DNA polymerase III theta subunit. Mueller GA, et al. J Bacteriol, 2005 Oct. PMID 16199579, Free PMC Article
    5. holE, the gene coding for the theta subunit of DNA polymerase III of Escherichia coli: characterization of a holE mutant and comparison with a dnaQ (epsilon-subunit) mutant. Slater SC, et al. J Bacteriol, 1994 Feb. PMID 8300534, Free PMC Article

    See all (66) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data show that the tau (tau), alpha (alpha), epsilon (epsilon), and theta (theta;) subunits of DNA Polymerase III can be assembled in vivo, yielding the trimeric tau3alpha3epsilon3theta;3 complex.
      Title: Simultaneous ternary extension of DNA catalyzed by a trimeric replicase assembled in vivo.
    2. A structural model of the alphaepsilontheta;:beta2 replicase complex reveals a compact, but still flexible, structure of the complex.
      Title: Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, epsilon, θ and β reveals a highly flexible arrangement of the proofreading domain.
    3. N-terminus verified by Edman degradation on complete protein
      Title: DNA polymerase III accessory proteins. V. Theta encoded by holE.
    4. The structure of theta in 60/40 (vol/vol) water-methanol is similar to that of HOT but differs significantly from a previously reported theta structure.
      Title: Nuclear magnetic resonance solution structure of the Escherichia coli DNA polymerase III theta subunit.
    5. evidence that hydrophobic interactions have a predominant contribution to the stability of the complex and that the association of epsilon186 with theta stabilized epsilon186
      Title: Application of electrospray ionization mass spectrometry to study the hydrophobic interaction between the epsilon and theta subunits of DNA polymerase III.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-directed DNA polymerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-directed DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA-templated DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in lagging strand elongation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in leading strand elongation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DNA polymerase III complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of DNA polymerase III, core complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of DNA polymerase III, core complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of replisome NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA polymerase III subunit theta

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1925108..1925338
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416356.1 DNA polymerase III subunit theta [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416356.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A829L8L5, A0AAN3SGW6
      Conserved Domains (1) summary
      PRK10969
      Location:1 → 74
      PRK10969; DNA polymerase III subunit theta; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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