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    holC DNA polymerase III subunit chi [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948787, updated on 3-Dec-2024

    Summary

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    Official Symbol
    holC
    Official Full Name
    DNA polymerase III subunit chi
    Primary source
    ECOCYC:EG11413
    Locus tag
    b4259
    See related
    ASAP:ABE-0013948
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4252
    Summary
    The preinitiation complex binds primed ssDNA and loads the beta processivity clamp. [More information is available at EcoCyc: EG11413].
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    Genomic context

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    Sequence:
    NC_000913.3 (4483837..4484280, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene inner membrane protein YjgN Neighboring gene valine--tRNA ligase Neighboring gene aminopeptidase A/I Neighboring gene protein YtgB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The Role of Replication Clamp-Loader Protein HolC of Escherichia coli in Overcoming Replication/Transcription Conflicts. Cooper DL, et al. mBio, 2021 Mar 9. PMID 33688004, Free PMC Article
    2. Site-directed mutagenesis of the χ subunit of DNA polymerase III and single-stranded DNA-binding protein of E. coli reveals key residues for their interaction. Naue N, et al. Nucleic Acids Res, 2011 Mar. PMID 20972214, Free PMC Article
    3. Characterization of the Escherichia coli XPD/Rad3 iron-sulfur helicase YoaA in complex with the DNA polymerase III clamp loader subunit chi (χ). Weeks-Pollenz SJ, et al. J Biol Chem, 2023 Jan. PMID 36509145, Free PMC Article
    4. Identification, molecular cloning and characterization of the gene encoding the chi subunit of DNA polymerase III holoenzyme of Escherichia coli. Carter JR, et al. Mol Gen Genet, 1993 Nov. PMID 8246893
    5. Genetic Analysis of DinG Family Helicase YoaA and Its Interaction with Replication Clamp Loader Protein HolC in Escherichia coli. Sutera VA, et al. J Bacteriol, 2021 Aug 20. PMID 34181484, Free PMC Article

    See all (92) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Characterization of the Escherichia coli XPD/Rad3 iron-sulfur helicase YoaA in complex with the DNA polymerase III clamp loader subunit chi (chi).
      Title: Characterization of the Escherichia coli XPD/Rad3 iron-sulfur helicase YoaA in complex with the DNA polymerase III clamp loader subunit chi (χ).
    2. Genetic Analysis of DinG Family Helicase YoaA and Its Interaction with Replication Clamp Loader Protein HolC in Escherichia coli.
      Title: Genetic Analysis of DinG Family Helicase YoaA and Its Interaction with Replication Clamp Loader Protein HolC in Escherichia coli.
    3. The Role of Replication Clamp-Loader Protein HolC of Escherichia coli in Overcoming Replication/Transcription Conflicts.
      Title: The Role of Replication Clamp-Loader Protein HolC of Escherichia coli in Overcoming Replication/Transcription Conflicts.
    4. This increased MMS sensitivity was not suppressed by the presence of multi-copy holC. These results indicate that yoaA is involved in MMS sensitivity and suggest that YoaA functions together with HolC.
      Title: Systematic identification of synthetic lethal mutations with reduced-genome Escherichia coli: synthetic genetic interactions among yoaA, xthA and holC related to survival from MMS exposure.
    5. Results support the idea that controlling the balance between SSB binding modes is critical for DNA Pol III holoenzyme stability.
      Title: ssb gene duplication restores the viability of ΔholC and ΔholD Escherichia coli mutants.
    6. evolutionary conserved residues of chi form a hydrophobic pocket to accommodate the ultimate two amino acids of single-stranded DNA-binding protein, P176 and F177.
      Title: Site-directed mutagenesis of the χ subunit of DNA polymerase III and single-stranded DNA-binding protein of E. coli reveals key residues for their interaction.
    7. N-terminus verified by Edman degradation on complete protein
      Title: DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-directed DNA polymerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-directed DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in DNA-templated DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated DNA replication initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within positive regulation of DNA-templated DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within response to radiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DNA polymerase III complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of DNA polymerase III, clamp loader complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of replisome NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA polymerase III subunit chi

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4483837..4484280 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418680.1 DNA polymerase III subunit chi [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418680.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A099AUD6, A0A0H3F3I3, A0A8E0FI71, A0AAN3V2C8
      Conserved Domains (1) summary
      PRK05728
      Location:1 → 147
      PRK05728; DNA polymerase III subunit chi; Validated
    Nucleotide Protein
    Heading Accession and Version

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