CBF2 Cbf2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CBF2
Official Full Name: Cbf2p
Primary source: SGD:S000003372
Locus tag: YGR140W
See related: AllianceGenome:SGD:S000003372; FungiDB:YGR140W; VEuPathDB:YGR140W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CEP2; CSL5; CTF14; NDC10
Summary: Enables DNA binding activity, bending and centromeric DNA binding activity. Involved in mitotic spindle elongation and septin ring assembly. Located in condensed chromosome, centromeric region and spindle midzone. Part of CBF3 complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CBF2 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (767429..770299)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation.
Title: Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation.
Exposure of bipartite hydrophobic signal triggers nuclear quality control of Ndc10 at the endoplasmic reticulum/nuclear envelope.
Title: Exposure of bipartite hydrophobic signal triggers nuclear quality control of Ndc10 at the endoplasmic reticulum/nuclear envelope.
Structure of yeast kinetochore Ndc10 DNA-binding domain reveals unexpected evolutionary relationship to tyrosine recombinases.
Title: Structure of yeast kinetochore Ndc10 DNA-binding domain reveals unexpected evolutionary relationship to tyrosine recombinases.
Localization of Cse4 to centromeres and the assembly activity depend on an evolutionarily conserved core motif in Scm3, but localization of the CBF3 subunit Ndc10 to centromeres does not depend on this motif.
Title: Scm3 is a centromeric nucleosome assembly factor.
Ndc10p is also essential for spindle pole body maturation and mitotic spindle integrity.
Title: Three-dimensional electron microscopy analysis of ndc10-1 mutant reveals an aberrant organization of the mitotic spindle and spindle pole body defects in Saccharomyces cerevisiae.
Ndc10p may play a direct role in maintaining spindle stability during anaphase and coordinates the completion of cell division after chromosome segregation
Title: The kinetochore protein Ndc10p is required for spindle stability and cytokinesis in yeast.
Phosphorylation of the middle region of Bir1 is required to bring Ndc10 to the spindle at anaphase, which is required for full spindle elongation.
Title: Phosphorylation of the chromosomal passenger protein Bir1 is required for localization of Ndc10 to the spindle during anaphase and full spindle elongation.
Ndc10/Scm3 binds to centromeric DNA, which is in turn essential for targeting Cse4 to centromeres.
Title: Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA binding, bending	IDA Inferred from Direct Assay more info	PubMed
enables centromeric DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in septin ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of CBF3 complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in condensed chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
located_in spindle midzone	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole body	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Cbf2p

NP_011656.3

Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

767429..770299

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181269.3 → NP_011656.3 TPA: Cbf2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011656.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUS0, E9P954, P32504

UniProtKB/TrEMBL

B3LI90, C8Z933, N1P5X8

Conserved Domains (2) summary

cd11602
Location:29 → 506

Ndc10; Ndc10 component of the yeast centromere-binding factor 3

pfam12550
Location:843 → 922

GCR1_C; Transcriptional activator of glycolytic enzymes