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    phoU negative regulator of the pho regulon [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948233, updated on 3-Dec-2024

    Summary

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    Official Symbol
    phoU
    Official Full Name
    negative regulator of the pho regulon
    Primary source
    ECOCYC:EG10735
    Locus tag
    b3724
    See related
    ASAP:ABE-0012177
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3717; phoT; pst
    Summary
    postulated that phoU encoded a repressor or a subunit of repressor for PhoB or one of the enzymes for converting phosphate to the hypothetical effector (corepressor), which activates a repressor for phoB . [More information is available at EcoCyc: EG10735].
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    Genomic context

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    Sequence:
    NC_000913.3 (3906853..3907578, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene beta-glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase Neighboring gene transcriptional antiterminator BglG Neighboring gene phosphate ABC transporter ATP binding subunit Neighboring gene phosphate ABC transporter membrane subunit PstA Neighboring gene phosphate ABC transporter membrane subunit PstC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Role of PhoU in phosphate transport and alkaline phosphatase regulation. Muda M, et al. J Bacteriol, 1992 Dec. PMID 1459954, Free PMC Article
    2. PhoU enhances the ability of extraintestinal pathogenic Escherichia coli strain CFT073 to colonize the murine urinary tract. Buckles EL, et al. Microbiology (Reading), 2006 Jan. PMID 16385125
    3. Regulation of the phosphate regulon in Escherichia coli K-12: regulation of the negative regulatory gene phoU and identification of the gene product. Nakata A, et al. J Bacteriol, 1984 Sep. PMID 6090402, Free PMC Article
    4. Purification of the phoU protein, a negative regulator of the pho regulon of Escherichia coli K-12. Surin BP, et al. J Bacteriol, 1986 Nov. PMID 3536855, Free PMC Article
    5. PhoU is a persistence switch involved in persister formation and tolerance to multiple antibiotics and stresses in Escherichia coli. Li Y, et al. Antimicrob Agents Chemother, 2007 Jun. PMID 17420206, Free PMC Article

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. PhoU interacts with PhoR, PstB, and metals to form a phosphate-signaling complex at the membrane.
      Title: The PhoU protein from Escherichia coli interacts with PhoR, PstB, and metals to form a phosphate-signaling complex at the membrane.
    2. The results indicate that PhoU controls the activity of the PstSCAB(2) transporter, as well as its abundance within the cell.
      Title: Employment of a promoter-swapping technique shows that PhoU modulates the activity of the PstSCAB2 ABC transporter in Escherichia coli.
    3. PhoU was found to interact with neither PhoB nor PhoR, while this acted as a phosphate starvation-inducible protein.
      Title: Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli.
    4. N-terminus verified by Edman degradation on complete protein
      Title: Purification of the phoU protein, a negative regulator of the pho regulon of Escherichia coli K-12.
    5. newly indentified persistence switch in persister formation and tolerance to antibiotics and stresses.
      Title: PhoU is a persistence switch involved in persister formation and tolerance to multiple antibiotics and stresses in Escherichia coli.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables manganese ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein homodimerization activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Process Evidence Code Pubs
    involved_in cellular response to antibiotic IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to heat IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to pH IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to phosphate starvation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to starvation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in intracellular phosphate ion homeostasis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in intracellular phosphate ion homeostasis TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in negative regulation of gene expression IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of gene expression TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in negative regulation of phosphate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within negative regulation of phosphate metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of phosphate metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of phosphate transmembrane transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in phosphate ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of cell growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    negative regulator of the pho regulon

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3906853..3907578 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418180.1 negative regulator of the pho regulon [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418180.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S0FF14
      Conserved Domains (1) summary
      PRK11115
      Location:1 → 236
      PRK11115; phosphate signaling complex protein PhoU
    Nucleotide Protein
    Heading Accession and Version

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