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    hyaB hydrogenase 1 large subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945580, updated on 3-Dec-2024

    Summary

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    Official Symbol
    hyaB
    Official Full Name
    hydrogenase 1 large subunit
    Primary source
    ECOCYC:EG10469
    Locus tag
    b0973
    See related
    ASAP:ABE-0003288
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0964
    Summary
    HyaB is co-exported bound to TAT substrate HyaA. [More information is available at EcoGene: EG10469]. HyaB is the large subunit of hydrogenase 1; it is thought to bind the Ni-Fe active site cofactor. [More information is available at EcoCyc: EG10469].
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    Genomic context

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    Sequence:
    NC_000913.3 (1033254..1035047)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene modulator of FtsH protease Neighboring gene tRNA-Ser Neighboring gene hydrogenase 1 small subunit Neighboring gene hydrogenase 1 cytochrome b subunit Neighboring gene putative hydrogenase 1 maturation protease HyaD Neighboring gene putative HyaA chaperone Neighboring gene protein HyaF

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structure-function relationships among the nickel-containing hydrogenases. Przybyla AE, et al. FEMS Microbiol Rev, 1992 Feb. PMID 1558764
    2. Carboxy-terminal processing of the large subunit of [NiFe] hydrogenases. Menon NK, et al. FEBS Lett, 1993 Sep 27. PMID 8405419
    3. Mechanism of hydrogen activation by [NiFe] hydrogenases. Evans RM, et al. Nat Chem Biol, 2016 Jan. PMID 26619250
    4. Response of hya expression to external pH in Escherichia coli. King PW, et al. J Bacteriol, 1999 Sep. PMID 10464194, Free PMC Article
    5. Importance of the Active Site "Canopy" Residues in an O(2)-Tolerant [NiFe]-Hydrogenase. Brooke EJ, et al. Biochemistry, 2017 Jan 10. PMID 28001048

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Studies indicate that Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, and the arginine/lysine substitution lowers the H2 oxidation rate by nearly 2 orders of magnitude.
      Title: Importance of the Active Site "Canopy" Residues in an O(2)-Tolerant [NiFe]-Hydrogenase.
    2. Crystal structure of dimeric membrane-bound O(2)-tolerant hydrogenase 1 from Escherichia coli in a 2:1 complex with its physiological partner, cytochrome b.
      Title: Crystal structure of the O(2)-tolerant membrane-bound hydrogenase 1 from Escherichia coli in complex with its cognate cytochrome b.
    3. Hydrogen-dependent reduction of benzyl viologen in hydrogenases HyaB, HybC and HybE is dependent on the [Fe-S]-cluster-containing small subunit.
      Title: Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulfur cluster-containing small subunit.
    4. Studies indicate that Hyd-1 and Hyd-2 are two H(2) uptake enzymes, while Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, Hyd-2 is encoded by genes hybO and hybC of the hybOABCDEFG operon.
      Title: How Escherichia coli is equipped to oxidize hydrogen under different redox conditions.
    5. N-terminus verified by Edman degradation on complete protein
      Title: Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables electron transfer activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ferredoxin hydrogenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrogenase (acceptor) activity IC
    Inferred by Curator
    more info
    		 PubMed 
    enables hydrogenase (acceptor) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nickel cation binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nickel cation binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within anaerobic electron transport chain IC
    Inferred by Curator
    more info
    		 PubMed 
    involved_in anaerobic electron transport chain IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in anaerobic respiration IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within anaerobic respiration IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to starvation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in fermentation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within fermentation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within hydrogen metabolic process IC
    Inferred by Curator
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of [Ni-Fe] hydrogenase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of [Ni-Fe] hydrogenase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of [Ni-Fe] hydrogenase complex RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    located_in membrane HDA PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in periplasmic side of plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    hydrogenase 1 large subunit
    NP_415492.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1033254..1035047
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415492.1 hydrogenase 1 large subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415492.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AAI9GXY8, A0AAN5ZI55
      Conserved Domains (1) summary
      PRK10170
      Location:1 → 597
      PRK10170; Ni/Fe-hydrogenase large subunit
    Nucleotide Protein
    Heading Accession and Version

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