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    glpF glycerol facilitator [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948422, updated on 3-Dec-2024

    Summary

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    Official Symbol
    glpF
    Official Full Name
    glycerol facilitator
    Primary source
    ECOCYC:EG10396
    Locus tag
    b3927
    See related
    ASAP:ABE-0012828
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3919
    Summary
    The glycerol facilitator, GlpF, allows the facilitated diffusion of glycerol across the inner membrane for utilization inside the cell. [More information is available at EcoCyc: EG10396].
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    Genomic context

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    Sequence:
    NC_000913.3 (4117245..4118090, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene fructose-1,6-bisphosphatase 2 Neighboring gene glycerol kinase Neighboring gene cell division factor ZapB Neighboring gene ribonuclease E inhibitor protein A

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Preparation of glycerol facilitator for protein structure and folding studies in solution. Manley D, et al. Methods Mol Biol, 2003. PMID 12824546
    2. Bioinformatics. Reality simulation--observe while it happens. Berendsen HJ. Science, 2001 Dec 14. PMID 11743188
    3. Role of C-terminal domain and transmembrane helices 5 and 6 in function and quaternary structure of major intrinsic proteins: analysis of aquaporin/glycerol facilitator chimeric proteins. Duchesne L, et al. J Biol Chem, 2002 Jun 7. PMID 11927589
    4. Water transporters: how so fast yet so selective? Law RJ, et al. Curr Biol, 2002 Apr 2. PMID 11937042
    5. Aquaglyceroporins: channel proteins with a conserved core, multiple functions, and variable surfaces. Engel A, et al. Int Rev Cytol, 2002. PMID 11952238

    See all (98) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Multiple pore lining residues modulate water permeability of GlpF.
      Title: Multiple pore lining residues modulate water permeability of GlpF.
    2. Mutation of tryptophan Trp219 affects the aqualgyeroporin GlpF activity and the stability of the active GlpF tetramer.
      Title: The GlpF residue Trp219 is part of an amino-acid cluster crucial for aquaglyceroporin oligomerization and function.
    3. Osmotic water permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations have been presented.
      Title: Computing osmotic permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations.
    4. Folding of the aquaglyceroporin GlpF determines its stability. (Review)
      Title: Folding and stability of the aquaglyceroporin GlpF: Implications for human aqua(glycero)porin diseases.
    5. TMAO increases the heat stability of the GF tetramer an average of 10 degrees C in the 4 detergents and also protects the protein from denaturation by SDS.
      Title: The effects of a protein osmolyte on the stability of the integral membrane protein glycerol facilitator.
    6. The tetrameric alpha-helical GlpF unfolds via a dimeric folding intermediate.
      Title: The tetrameric α-helical membrane protein GlpF unfolds via a dimeric folding intermediate.
    7. GlpF exists as a stably folded tetramer in detergent solutions of neutral dodecyl maltoside and in zwitterionic lysomyristoylphosphatidylcholine.
      Title: Stability of the glycerol facilitator in detergent solutions.
    8. GlpF is found to be highly permeable for small solutes and permeable for larger solutes.
      Title: Mechanism of selectivity in aquaporins and aquaglyceroporins.
    9. analysis of of Escherichia coli aquaporin AqpZ and GlpF single-channel water permeabilities
      Title: Single-channel water permeabilities of Escherichia coli aquaporins AqpZ and GlpF.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables channel activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glycerol channel activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables glycerol channel activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables glycerol transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    NOT enables water transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cellular response to mercury ion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in glycerol transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in glycerol transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in glycerol transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within glycerol transmembrane transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    NOT involved_in water transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    glycerol facilitator

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4117245..4118090 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418362.1 glycerol facilitator [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418362.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6C9Q6C8, A0A7D7PKY3
      Conserved Domains (1) summary
      cd00333
      Location:10 → 254
      MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...
    Nucleotide Protein
    Heading Accession and Version

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