RPN12 proteasome regulatory particle lid subunit RPN12 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPN12
Official Full Name: proteasome regulatory particle lid subunit RPN12
Primary source: SGD:S000001948
Locus tag: YFR052W
See related: AllianceGenome:SGD:S000001948; FungiDB:YFR052W; VEuPathDB:YFR052W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: NIN1
Summary: Involved in ubiquitin-dependent protein catabolic process. Located in proteasome storage granule. Part of proteasome regulatory particle, lid subcomplex. Orthologous to human PSMD8 (proteasome 26S subunit, non-ATPase 8). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RPN12 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 1

Sequence:: Chromosome: VI; NC_001138.5 (252505..253329)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Assimilation of the final lid subunit of the 26S proteasome, Rpn12, triggers a large-scale conformational remodeling of the nascent lid that drives regulatory particles assembly, in part by relieving steric clash with the base.
Title: A Single α Helix Drives Extensive Remodeling of the Proteasome Lid and Completion of Regulatory Particle Assembly.
Data show that proteasome subunits Rpt1, Rpt4, Rpn8, Rpn12, Pre6, and Pre10 are recruited to GAL10 rapidly upon galactose induction.
Title: Similar temporal and spatial recruitment of native 19S and 20S proteasome subunits to transcriptionally active chromatin.
Mass spectra of the intact lid complex from Saccharomyces cerevisiae show that eight of the nine subunits are present stoichiometrically and that a stable tetrameric subcomplex forms in solution.
Title: Structural organization of the 19S proteasome lid: insights from MS of intact complexes.
19S RP modulates SAGA complex using its ATPase components, thereby facilitating subsequent transcription events at promoters.
Title: The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome complex	IEA Inferred from Electronic Annotation more info
part_of proteasome regulatory particle	IEA Inferred from Electronic Annotation more info
part_of proteasome regulatory particle, lid subcomplex	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome regulatory particle, lid subcomplex	IDA Inferred from Direct Assay more info	PubMed
located_in proteasome storage granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: proteasome regulatory particle lid subunit RPN12

NP_116710.1

Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001138.5 Reference assembly

Range

252505..253329

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180017.1 → NP_116710.1 TPA: proteasome regulatory particle lid subunit RPN12 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_116710.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTT5, P32496

UniProtKB/TrEMBL

A7A292, B3LUR3, B5VI77, C7GYF5, C8Z7Y7, G2WDJ3, N1PA14

Conserved Domains (1) summary

cl24019
Location:122 → 226

CSN8_PSD8_EIF3K; CSN8/PSMD8/EIF3K family