U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    nth endonuclease III [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947122, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    nth
    Official Full Name
    endonuclease III
    Primary source
    ECOCYC:EG10662
    Locus tag
    b1633
    See related
    ASAP:ABE-0005463
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1629
    Summary
    Nth is specific for damaged pyrimidine sites, particularly Thy. [More information is available at EcoGene: EG10662]. Endonuclease III or Nth is a DNA glycosylase that recognizes pyrimidine residues damaged by ring saturation, ring fragmentation or ring contraction. [More information is available at EcoCyc: EG10662].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (1711523..1712158)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene SoxR [2Fe-2S] reducing system protein RsxD Neighboring gene SoxR [2Fe-2S] reducing system protein RsxG Neighboring gene SoxR [2Fe-2S] reducing system protein RsxE Neighboring gene dipeptide/tripeptide:H(+) symporter DtpA Neighboring gene glutathione S-transferase GstA

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Role of endonuclease III enzymes in uracil repair. Yang Y, et al. Mutat Res, 2019 Jan. PMID 30590231, Free PMC Article
    2. Novel substrates of Escherichia coli nth protein and its kinetics for excision of modified bases from DNA damaged by free radicals. Dizdaroglu M, et al. Biochemistry, 2000 May 9. PMID 10820032
    3. Enzymatic processing of uracil glycol, a major oxidative product of DNA cytosine. Purmal AA, et al. J Biol Chem, 1998 Apr 17. PMID 9545349
    4. Excision of oxidative cytosine modifications from gamma-irradiated DNA by Escherichia coli endonuclease III and human whole-cell extracts. Wagner JR, et al. Anal Biochem, 1996 Jan 1. PMID 8789150
    5. In vitro studies on DNA-photosensitization by different drug stereoisomers. Lhiaubet-Vallet V, et al. Toxicol In Vitro, 2003 Oct-Dec. PMID 14599458

    See all (73) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. WT NTHL1 (human) and Nth (E. coli) are remarkably alike with respect to specificity of glycosylase reaction, and although NTHL1 is a much slower enzyme than Nth, the tighter binding of NTHL1 compensates, resulting in similar kcat/Kd values for both enzymes with each of the substrates tested. EcoNth Thr121 residue influences enzyme binding to DNA, as binding is significantly reduced with the Thr121Ala variant.
      Title: Probing the activity of NTHL1 orthologs by targeting conserved amino acid residues.
    2. A possible pathway is proposed through which charge can travel between the DNA and the iron sulfur cluster of EndoIII, as a first step in damage detection.
      Title: Mutants of the base excision repair glycosylase, endonuclease III: DNA charge transport as a first step in lesion detection.
    3. N-terminus verified by Edman degradation on complete protein
      Title: Purification and characterization of Escherichia coli endonuclease III from the cloned nth gene.
    4. Endonuclease III does not protect E.coli against possible mutations attributable to formamidopyrimidine lesions.
      Title: Excision of formamidopyrimidine lesions by endonucleases III and VIII is not a major DNA repair pathway in Escherichia coli.
    5. Electrochemical measurement of endonuclease III using DNA-modified electrodes demonstrates that this base excision repair enzyme containing a [4Fe-4S] cofactor is redox-active when bound to DNA.
      Title: DNA-bound redox activity of DNA repair glycosylases containing [4Fe-4S] clusters.
    Submit: New GeneRIF Correction

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA N-glycosylase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA N-glycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-(apurinic or apyrimidinic site) endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables class I DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, acting on glycosyl bonds IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables uracil DNA N-glycosylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in base-excision repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in base-excision repair, AP site formation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within base-excision repair, AP site formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within base-excision repair, AP site formation via deaminated base removal IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within cellular response to UV IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in response to stress IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    endonuclease III

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1711523..1712158
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416150.1 endonuclease III [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416150.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AID0
      Conserved Domains (1) summary
      PRK10702
      Location:1 → 211
      PRK10702; endonuclease III; Provisional
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.