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    TOF2 Tof2p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853880, updated on 9-Dec-2024

    Summary

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    Official Symbol
    TOF2
    Official Full Name
    Tof2p
    Primary source
    SGD:S000001718
    Locus tag
    YKR010C
    See related
    AllianceGenome:SGD:S000001718; FungiDB:YKR010C; VEuPathDB:YKR010C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables phosphatase activator activity. Involved in protein localization to nucleolar rDNA repeats; rDNA chromatin condensation; and rDNA heterochromatin formation. Located in nucleolus. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See TOF2 in Genome Data Viewer
    Location:
    chromosome: XI
    Exon count:
    1
    Sequence:
    Chromosome: XI; NC_001143.9 (458924..461239, complement)

    Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene bifunctional hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase FOX2 Neighboring gene tRNA-Leu Neighboring gene chromatin-associated RNAPIII regulator FPT1 Neighboring gene sterol-binding protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001143.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Reversible Top1 cleavage complexes are stabilized strand-specifically at the ribosomal replication fork barrier and contribute to ribosomal DNA stability. Krawczyk C, et al. Nucleic Acids Res, 2014 Apr. PMID 24574527, Free PMC Article
    2. The polySUMOylation axis promotes nucleolar release of Tof2 for mitotic exit. Gutierrez-Morton E, et al. Cell Rep, 2024 Jul 23. PMID 39002125, Free PMC Article
    3. A nucleolus-localized activator of Cdc14 phosphatase supports rDNA segregation in yeast mitosis. Geil C, et al. Curr Biol, 2008 Jul 8. PMID 18595708
    4. Identification and characterization of the genes for two topoisomerase I-interacting proteins from Saccharomyces cerevisiae. Park H, et al. Yeast, 1999 Jan 15. PMID 10028183
    5. Mechanism of Regulation of Intrachromatid Recombination and Long-Range Chromosome Interactions in Saccharomyces cerevisiae. Zaman S, et al. Mol Cell Biol, 2016 May 15. PMID 26951198, Free PMC Article

    See all (66) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The authors show that Siz2 is enriched at the replication fork barrier (RFB) in the ribosomal DNA (rDNA) and also controls the homeostasis of Tof2 protein. siz2Delta resulted in increased accumulation of total Tof2 in the cell and a consequent increase in the enrichment of Tof2 at the rDNA.
      Title: Siz2 Prevents Ribosomal DNA Recombination by Modulating Levels of Tof2 in Saccharomyces cerevisiae.
    2. Ctf4-dependent recruitment of Dna2/Tof2 couples other processes to DNA synthesis, including rDNA copy-number regulation.
      Title: Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase.
    3. These data support a model whereby Tof2 coordinates the biphasic release of Cdc14 during anaphase by restraining a population of Cdc14 in the nucleolus after activation of the Cdc14 early anaphase release (FEAR) network, for subsequent release by the MEN.
      Title: Putting the brake on FEAR: Tof2 promotes the biphasic release of Cdc14 phosphatase during mitotic exit.
    4. collaborates with Cdc14 specifically in rDNA segregation, presumably by targeting Cdc14 phosphatase activity to the nucleolus during anaphase to support resolution and compaction of this repetitive and highly transcribed DNA locus
      Title: A nucleolus-localized activator of Cdc14 phosphatase supports rDNA segregation in yeast mitosis.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables phosphatase activator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables phosphatase activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables rDNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in nucleolus organization IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in nucleolus organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein localization to nucleolar rDNA repeats IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rDNA chromatin condensation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rDNA heterochromatin formation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in rDNA heterochromatin formation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rDNA heterochromatin formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in mitochondrion HDA PubMed 
    is_active_in nucleolus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Tof2p
    NP_012935.3
    • Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001143.9 Reference assembly

      Range
      458924..461239 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179800.3 → NP_012935.3  TPA: Tof2p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012935.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VX76, Q02208
      UniProtKB/TrEMBL
      B3LR95, C7GNP7
      Conserved Domains (1) summary
      pfam10407
      Location:83 → 153
      Cytokin_check_N; Cdc14 phosphatase binding protein N-terminus
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q02208.1

    Gene LinkOut

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