RPG1 translation initiation factor eIF3 core subunit a [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPG1
Official Full Name: translation initiation factor eIF3 core subunit a
Primary source: SGD:S000000283
Locus tag: YBR079C
See related: AllianceGenome:SGD:S000000283; FungiDB:YBR079C; VEuPathDB:YBR079C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TIF32
Summary: Enables translation initiation factor activity. Involved in translation reinitiation. Located in cytoplasmic stress granule and incipient cellular bud site. Part of eukaryotic translation initiation factor 3 complex and multi-eIF complex. Orthologous to human EIF3A (eukaryotic translation initiation factor 3 subunit A). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RPG1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (395383..398277, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rps3 and eIF3 closely co-operate to control translation termination and stop codon readthrough.
Title: uS3/Rps3 controls fidelity of translation termination and programmed stop codon readthrough in co-operation with eIF3.
Cryo-EM structures indicate eukaryotic initiation factor eIF3 as a molecular embracer of the 40S subunit, and the binding of eIF1 and eIF5 further fortified the compact architecture of eIF3.
Title: Binding of eIF3 in complex with eIF5 and eIF1 to the 40S ribosomal subunit is accompanied by dramatic structural changes.
Mutations in the Rpg1-3 protein render not only eIF3a but, more importantly, also the eIF3 core complex prone to aggregation that is potentiated by the limited availability of Hsp70 and Hsp40 chaperones.
Title: An aggregation-prone mutant of eIF3a forms reversible assemblies escaping spatial control in exponentially growing yeast cells.
The interaction between the flexible RPS0A-C-terminal tail and residues 200-400 of the TIF32-N-terminal domain significantly stimulates attachment of eIF3 and its associated eIFs to small ribosomal subunits in vivo.
Title: Small ribosomal protein RPS0 stimulates translation initiation by mediating 40S-binding of eIF3 via its direct contact with the eIF3a/TIF32 subunit.
Data suggest that the eIF3b-eIF3j/eIF3a module binds near the mRNA entry channel and regulates the transition between scanning-conducive and initiation-competent conformations of the preinitiation complex.
Title: The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons.
eIF3 is retained on ribosomes throughout uORF1 translation and, interacts with its 5' enhancer at the mRNA exit channel to stabilize mRNA association with post-termination 40S subunits and enable resumption of scanning for reinitiation downstream.
Title: eIF3a cooperates with sequences 5' of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA.
The monoclonal antibody PK1 recognized eIFa, the mammalian orthologue of yeast Rpg1, in CHO cells.
Title: Specification of the monoclonal antibody PK1 reactivity in Chinese hamster ovary cells.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables translation initiation factor activity	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cytoplasmic translational initiation	IEA Inferred from Electronic Annotation more info
involved_in formation of cytoplasmic translation initiation complex	IBA Inferred from Biological aspect of Ancestor more info
involved_in formation of cytoplasmic translation initiation complex	IEA Inferred from Electronic Annotation more info
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translation reinitiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translation reinitiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translational initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in translational initiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
part_of eukaryotic 43S preinitiation complex	IEA Inferred from Electronic Annotation more info
part_of eukaryotic 48S preinitiation complex	IEA Inferred from Electronic Annotation more info
part_of eukaryotic translation initiation factor 3 complex	IDA Inferred from Direct Assay more info	PubMed
part_of eukaryotic translation initiation factor 3 complex	IEA Inferred from Electronic Annotation more info
part_of eukaryotic translation initiation factor 3 complex	IPI Inferred from Physical Interaction more info	PubMed
part_of eukaryotic translation initiation factor 3 complex, eIF3e	IBA Inferred from Biological aspect of Ancestor more info
part_of eukaryotic translation initiation factor 3 complex, eIF3m	IBA Inferred from Biological aspect of Ancestor more info
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
part_of multi-eIF complex	IBA Inferred from Biological aspect of Ancestor more info
part_of multi-eIF complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: translation initiation factor eIF3 core subunit a

NP_009635.1

eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; essential for loading mRNA onto ribosome at entry and exit channels of preinitiation complex; involved in translation reinitiation; eIF3 is also involved in programmed stop codon readthrough

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

395383..398277 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178427.1 → NP_009635.1 TPA: translation initiation factor eIF3 core subunit a [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009635.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQ78, P38249

UniProtKB/TrEMBL

A6ZL23, N1P8C7

Conserved Domains (2) summary

smart00088
Location:419 → 495

PINT; motif in proteasome subunits, Int-6, Nip-1 and TRIP-15

pfam17380
Location:595 → 862

DUF5401; Family of unknown function (DUF5401)