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    mrdA peptidoglycan DD-transpeptidase MrdA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945240, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mrdA
    Official Full Name
    peptidoglycan DD-transpeptidase MrdA
    Primary source
    ECOCYC:EG10606
    Locus tag
    b0635
    See related
    ASAP:ABE-0002178
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0628; pbpA
    Summary
    Penicillin binding protein 2 (PBP2) is responsible for maintaining the rod cell shape and mecillinam sensitivity in Escherichia coli along with the product of the mrdB gene, RodA . mrdA and mrdB share a transcription unit . [More information is available at EcoCyc: EG10606].
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    Genomic context

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    Sequence:
    NC_000913.3 (666316..668217, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene rare lipoprotein RlpA Neighboring gene peptidoglycan glycosyltransferase MrdB Neighboring gene 23S rRNA m(3)psi1915 methyltransferase Neighboring gene ribosomal silencing factor RsfS

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Comparative potency of mecillinam and other beta-lactam antibiotics against Escherichia coli strains producing different beta-lactamases. Sougakoff W, et al. J Antimicrob Chemother, 2000 Sep. PMID 11051618
    2. In vitro and in vivo activities of AM-112, a novel oxapenem. Jamieson CE, et al. Antimicrob Agents Chemother, 2003 May. PMID 12709336, Free PMC Article
    3. Target affinities of faropenem to and its impact on the morphology of gram-positive and gram-negative bacteria. Dalhoff A, et al. Chemotherapy, 2003 Jul. PMID 12886052
    4. A mecillinam-sensitive peptidoglycan crosslinking reaction in Escherichia coli. Ishino F, et al. Biochem Biophys Res Commun, 1982 Dec 15. PMID 6297485
    5. Mode of action of azthreonam. Georgopapadakou NH, et al. Antimicrob Agents Chemother, 1982 Jun. PMID 6180685, Free PMC Article

    See all (85) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. mutations in PBP2 reduce susceptibility to carbapenems in E. coli clinical isolates that also have alterations of PBP3 and express the NDM-1 beta-lactamase
      Title: Identification of Mutations in the mrdA Gene Encoding PBP2 That Reduce Carbapenem and Diazabicyclooctane Susceptibility of Escherichia coli Clinical Isolates with Mutations in ftsI (PBP3) and Which Carry bla(NDM-1).
    2. Mutations in mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells.
      Title: Mutations in cell elongation genes mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells.
    3. Data show that MreB and FtsZ direct the synthesis of lateral cell wall via independent pathways that require PBP 2.
      Title: In Escherichia coli, MreB and FtsZ direct the synthesis of lateral cell wall via independent pathways that require PBP 2.
    4. Cells lacking mrdA were fully viable and propagated as small spheres under conditions of slow mass increase but formed large nondividing spheroids with noncanonical FtsZ assembly patterns at higher mass doubling rates.
      Title: Conditional lethality, division defects, membrane involution, and endocytosis in mre and mrd shape mutants of Escherichia coli.
    5. The morphological effects and lysis were also eliminated entirely when overproduction of penicillin-binding protein 2 (PBP 2) and variants was conducted.
      Title: Overproduction of penicillin-binding protein 2 and its inactive variants causes morphological changes and lysis in Escherichia coli.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables carboxypeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables penicillin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables penicillin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables penicillin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables penicillin binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables peptidoglycan L,D-transpeptidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables peptidoglycan L,D-transpeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables serine-type D-Ala-D-Ala carboxypeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables serine-type D-Ala-D-Ala carboxypeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables serine-type D-Ala-D-Ala carboxypeptidase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables serine-type D-Ala-D-Ala carboxypeptidase activity ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cell wall organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in cell wall organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cell wall organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in peptidoglycan biosynthetic process IC
    Inferred by Curator
    more info
    		 PubMed 
    acts_upstream_of_or_within peptidoglycan biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in peptidoglycan biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in peptidoglycan biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within peptidoglycan biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in peptidoglycan biosynthetic process NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of cell shape IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of cell shape IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of cell shape IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of cell shape NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within response to antibiotic IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in outer membrane-bounded periplasmic space ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    located_in plasma membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    peptidoglycan DD-transpeptidase MrdA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      666316..668217 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415168.1 peptidoglycan DD-transpeptidase MrdA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415168.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2W6PGZ2, A0A7A6VUS0, A0AAN3LFR0
      Conserved Domains (1) summary
      PRK10795
      Location:1 → 632
      PRK10795; penicillin-binding protein 2; Provisional
    Nucleotide Protein
    Heading Accession and Version

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