NONO non-POU domain containing octamer binding [Homo sapiens (human)] - Gene

Summary

Official Symbol: NONOprovided by HGNC
Official Full Name: non-POU domain containing octamer bindingprovided by HGNC
Primary source: HGNC:HGNC:7871
See related: Ensembl:ENSG00000147140 MIM:300084; AllianceGenome:HGNC:7871
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
Summary: This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
Expression: Ubiquitous expression in ovary (RPKM 81.7), lymph node (RPKM 64.3) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: Xq13.1

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	X	NC_000023.11 (71283635..71301168)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	X	NC_060947.1 (69717744..69735278)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	X	NC_000023.10 (70503485..70521018)

Chromosome X - NC_000023.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nucleolar detention of NONO shields DNA double-strand breaks from aberrant transcripts.
Title: Nucleolar detention of NONO shields DNA double-strand breaks from aberrant transcripts.
NONO promotes gallbladder cancer cell proliferation by enhancing oncogenic RNA splicing of DLG1 through interaction with IGF2BP3/RBM14.
Title: NONO promotes gallbladder cancer cell proliferation by enhancing oncogenic RNA splicing of DLG1 through interaction with IGF2BP3/RBM14.
IKZF4/NONO-RAB11FIP3 axis promotes immune evasion in gastric cancer via facilitating PD-L1 endosome recycling.
Title: IKZF4/NONO-RAB11FIP3 axis promotes immune evasion in gastric cancer via facilitating PD-L1 endosome recycling.
The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA.
Title: The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA.
Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/beta-catenin pathways in human breast cancer cells.
Title: Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/β-catenin pathways in human breast cancer cells.
NONO enhances mRNA processing of super-enhancer-associated GATA2 and HAND2 genes in neuroblastoma.
Title: NONO enhances mRNA processing of super-enhancer-associated GATA2 and HAND2 genes in neuroblastoma.
A novel NONO variant that causes developmental delay and cardiac phenotypes.
Title: A novel NONO variant that causes developmental delay and cardiac phenotypes.
NONO regulates multiple cytokine production in sepsis via the ERK1/2 signaling pathway.
Title: NONO regulates multiple cytokine production in sepsis via the ERK1/2 signaling pathway.
Targeting the splicing factor NONO inhibits GBM progression through GPX1 intron retention.
Title: Targeting the splicing factor NONO inhibits GBM progression through GPX1 intron retention.
Molecular Modelling of NONO and SFPQ Dimerization Process and RNA Recognition Mechanism.
Title: Molecular Modelling of NONO and SFPQ Dimerization Process and RNA Recognition Mechanism.

Submit: New GeneRIF Correction See all GeneRIFs (92)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description	Tests
Syndromic X-linked intellectual disability 34 MedGen: C4225417 OMIM: 300967 GeneReviews: Not available	Compare labs

Copy number response

Description
Copy number response Triplosensitivity No evidence available (Last evaluated 2024-07-24) ClinGen Genome Curation Page Haploinsufficency Sufficient evidence for dosage pathogenicity (Last evaluated 2024-07-24) ClinGen Genome Curation PagePubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
Knockdown of NONO by shRNA enhances HIV-1 replication in both Jurkat cells and primary CD4+ T-cells	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Gag-Pol	gag-pol	Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Nef	nef	Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Pr55(Gag)	gag	Knockdown of NONO expression by shRNA enhances both HIV-1 Gag expression in Jurkat cells and the released p24 levels from these cells	PubMed
	gag	Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Rev	rev	Co-immunoprecipitation analysis demonstrates that endogenous RBM14 associates with HIV-1 Rev, XPO1, and p54/NRB in cells	PubMed
	rev	HIV-1 Rev interacting protein, non-POU domain containing, octamer-binding (NONO), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with NONO is increased by RRE	PubMed
Vpr	vpr	A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies upregulation of non-POU domain containing octamer-binding protein (NONO) expression by HIV-1 Vpr in Vpr transduced macrophages	PubMed
capsid	gag	Knockdown of NONO expression by shRNA enhances both HIV-1 Gag expression in Jurkat cells and the released p24 levels from these cells	PubMed
retropepsin	gag-pol	Positional proteomics analysis identifies the cleavage of human non-POU domain containing, octamer-binding (NONO) at amino acid residues 54-55, 274-275, and 277-278 by the HIV-1 protease	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

GDB:434012 (e-PCR)
- UniSTS:157204

D10S275 (e-PCR)
- UniSTS:147992

NoName (e-PCR)
- UniSTS:481582

DXS1500 (e-PCR)
- UniSTS:99633

D8S2279 (e-PCR)
- UniSTS:473907

DXS9791 (e-PCR)
- UniSTS:9273

L17709 (e-PCR)
- UniSTS:42599

D10S16 (e-PCR)
- UniSTS:155756

D1S1425 (e-PCR)
- UniSTS:149621

DXS7556 (e-PCR)
- UniSTS:26513

RH18146 (e-PCR)
- UniSTS:209598

D5S1597E (e-PCR)
- UniSTS:151019

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	HDA more info	PubMed
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin binding	ISS Inferred from Sequence or Structural Similarity more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in activation of innate immune response	IDA Inferred from Direct Assay more info	PubMed
involved_in circadian rhythm	ISS Inferred from Sequence or Structural Similarity more info
involved_in innate immune response	IEA Inferred from Electronic Annotation more info
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of circadian rhythm	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of RNA polymerase II transcription regulator complex	ISS Inferred from Sequence or Structural Similarity more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in fibrillar center	IDA Inferred from Direct Assay more info
located_in membrane	HDA more info	PubMed
located_in nuclear matrix	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
is_active_in paraspeckles	IDA Inferred from Direct Assay more info	PubMed
located_in paraspeckles	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: non-POU domain-containing octamer-binding protein

Names: 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; non-POU domain-containing octamer (ATGCAAAT) binding protein; p54(nrb); protein phosphatase 1, regulatory subunit 114

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_046742.1 RefSeqGene

Range

5444..22977

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001145408.2 → NP_001138880.1 non-POU domain-containing octamer-binding protein isoform 1

See identical proteins and their annotated locations for NP_001138880.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).

Source sequence(s)

BC002364, BC003129, DA116840

Consensus CDS

CCDS14410.1

UniProtKB/Swiss-Prot

B7Z4C2, D3DVV4, F5GYZ3, O00201, P30807, Q12786, Q15233, Q9BQC5

UniProtKB/TrEMBL

A0A0S2Z4Z9, A8K525

Related

ENSP00000410299.2, ENST00000420903.6

Conserved Domains (3) summary

cd12946
Location:219 → 311

NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins

cd12588
Location:73 → 143

RRM1_p54nrb; RNA recognition motif 1 (RRM1) found in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)

cl17169
Location:149 → 228

RRM_SF; RNA recognition motif (RRM) superfamily
NM_001145409.2 → NP_001138881.1 non-POU domain-containing octamer-binding protein isoform 1

See identical proteins and their annotated locations for NP_001138881.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).

Source sequence(s)

BC002364, BC012141, DA116840

Consensus CDS

CCDS14410.1

UniProtKB/Swiss-Prot

B7Z4C2, D3DVV4, F5GYZ3, O00201, P30807, Q12786, Q15233, Q9BQC5

UniProtKB/TrEMBL

A0A0S2Z4Z9, A8K525

Related

ENSP00000362947.1, ENST00000373841.5

Conserved Domains (3) summary

cd12946
Location:219 → 311

NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins

cd12588
Location:73 → 143

RRM1_p54nrb; RNA recognition motif 1 (RRM1) found in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)

cl17169
Location:149 → 228

RRM_SF; RNA recognition motif (RRM) superfamily
NM_001145410.2 → NP_001138882.1 non-POU domain-containing octamer-binding protein isoform 2

See identical proteins and their annotated locations for NP_001138882.1

Status: REVIEWED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

Source sequence(s)

AK297144, BC002364

Consensus CDS

CCDS55445.1

UniProtKB/TrEMBL

A8K525

Related

ENSP00000441364.1, ENST00000535149.5

Conserved Domains (2) summary

cd12946
Location:130 → 222

NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins

cl17169
Location:60 → 139

RRM_SF; RNA recognition motif (RRM) superfamily
NM_007363.5 → NP_031389.3 non-POU domain-containing octamer-binding protein isoform 1

See identical proteins and their annotated locations for NP_031389.3

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).

Source sequence(s)

BC002364, BC028299, DA116840

Consensus CDS

CCDS14410.1

UniProtKB/Swiss-Prot

B7Z4C2, D3DVV4, F5GYZ3, O00201, P30807, Q12786, Q15233, Q9BQC5

UniProtKB/TrEMBL

A0A0S2Z4Z9, A8K525

Related

ENSP00000276079.8, ENST00000276079.13

Conserved Domains (3) summary

cd12946
Location:219 → 311

NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins

cd12588
Location:73 → 143

RRM1_p54nrb; RNA recognition motif 1 (RRM1) found in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)

cl17169
Location:149 → 228

RRM_SF; RNA recognition motif (RRM) superfamily