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    BTF3 basic transcription factor 3 [ Homo sapiens (human) ]

    Gene ID: 689, updated on 9-Dec-2024

    Summary

    Official Symbol
    BTF3provided by HGNC
    Official Full Name
    basic transcription factor 3provided by HGNC
    Primary source
    HGNC:HGNC:1125
    See related
    Ensembl:ENSG00000145741 MIM:602542; AllianceGenome:HGNC:1125
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    NACB; BTF3a; BTF3b; BETA-NAC
    Summary
    This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in ovary (RPKM 354.7), bone marrow (RPKM 186.5) and 25 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See BTF3 in Genome Data Viewer
    Location:
    5q13.2
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 5 NC_000005.10 (73498442..73505667)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 5 NC_060929.1 (73979765..73986989)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 5 NC_000005.9 (72794267..72801492)

    Chromosome 5 - NC_000005.10Genomic Context describing neighboring genes Neighboring gene long intergenic non-protein coding RNA 1386 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr5:72783409-72784169 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 22657 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr5:72794255-72795065 Neighboring gene BTF3 divergent transcript Neighboring gene FUN14 domain containing 2 pseudogene 1 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 16090 Neighboring gene ankyrin repeat family A member 2 Neighboring gene H3K27ac hESC enhancers GRCh37_chr5:72860724-72861278 and GRCh37_chr5:72861279-72861833

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    Knockdown of basic transcription factor 3 (BTF3) by shRNA library screening inhibits HIV-1 replication in cultured Jurkat T-cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    integrase gag-pol Using acetylated HIV-1 IN as bait in yeast two-hybrid screening identifies transcription regulatory and chromatin remodeling factors LEDGF/p75, KAP1, BTF3b, THRAP3, and HMGN2 as IN-binding partners PubMed

    Go to the HIV-1, Human Interaction Database

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables RNA binding HDA PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in in utero embryonic development IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in negative regulation of protein localization to endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
     
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
     
    part_of nascent polypeptide-associated complex IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    part_of nascent polypeptide-associated complex IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    located_in nucleus ISS
    Inferred from Sequence or Structural Similarity
    more info
     

    General protein information

    Preferred Names
    transcription factor BTF3
    Names
    NAC-beta
    RNA polymerase B transcription factor 3
    nascent polypeptide-associated complex subunit beta
    nascent-polypeptide-associated complex beta polypeptide

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001037637.2NP_001032726.1  transcription factor BTF3 isoform A

      See identical proteins and their annotated locations for NP_001032726.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (A).
      Source sequence(s)
      AC099522, BG182166, BU661989, BX359147
      Consensus CDS
      CCDS34185.1
      UniProtKB/Swiss-Prot
      A8K510, P20290, Q13893, Q76M56
      Related
      ENSP00000369965.3, ENST00000380591.8
      Conserved Domains (1) summary
      pfam01849
      Location:87140
      NAC; NAC domain
    2. NM_001207.5NP_001198.2  transcription factor BTF3 isoform B

      See identical proteins and their annotated locations for NP_001198.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in translation initiation at a downstream AUG and a protein (isoform B) with a shorter N-terminus, compared to isoform A.
      Source sequence(s)
      AC099522, BC008062, BG182166, BU661989
      Consensus CDS
      CCDS4019.1
      UniProtKB/TrEMBL
      A0A7I2V3T6
      Related
      ENSP00000338516.8, ENST00000335895.12
      Conserved Domains (1) summary
      cd22055
      Location:9125
      NAC_BTF3; basal transcription factor BTF3
    3. NM_001393652.1NP_001380581.1  transcription factor BTF3 isoform C

      Status: REVIEWED

      Source sequence(s)
      AC099522
    4. NM_001393653.1NP_001380582.1  transcription factor BTF3 isoform D

      Status: REVIEWED

      Source sequence(s)
      AC099522
      UniProtKB/TrEMBL
      A0A7I2YQL2
      Related
      ENSP00000503967.1, ENST00000676862.1

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000005.10 Reference GRCh38.p14 Primary Assembly

      Range
      73498442..73505667
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060929.1 Alternate T2T-CHM13v2.0

      Range
      73979765..73986989
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)