MCM2 MCM DNA helicase complex subunit MCM2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MCM2
Official Full Name: MCM DNA helicase complex subunit MCM2
Primary source: SGD:S000000119
Locus tag: YBL023C
See related: AllianceGenome:SGD:S000000119; FungiDB:YBL023C; VEuPathDB:YBL023C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA replication origin binding activity and chromatin binding activity. Contributes to single-stranded DNA binding activity and single-stranded DNA helicase activity. Involved in DNA metabolic process. Located in cytoplasm and nucleus. Part of MCM complex and nuclear lumen. Human ortholog(s) of this gene implicated in Alzheimer's disease and autosomal dominant nonsyndromic deafness 70. Orthologous to human MCM2 (minichromosome maintenance complex component 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MCM2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (174920..177526, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Functional control of Eco1 through the MCM complex in sister chromatid cohesion.
Title: Functional control of Eco1 through the MCM complex in sister chromatid cohesion.
DNA replication origins retain mobile licensing proteins.
Title: DNA replication origins retain mobile licensing proteins.
Antagonistic control of DDK binding to licensed replication origins by Mcm2 and Rad53.
Title: Antagonistic control of DDK binding to licensed replication origins by Mcm2 and Rad53.
The structures of the replication complex and the Mcm2-7 double hexamer on DNA suggest a completely unanticipated process for formation of bidirectional replication forks at origins. (Review)
Title: The Eukaryotic CMG Helicase at the Replication Fork: Emerging Architecture Reveals an Unexpected Mechanism.
ur results support a model whereby parental (H3-H4)2 complexes displaced from nucleosomes by DNA unwinding at replication forks are transferred by the CMG-Ctf4-Pol alpha complex to lagging-strand DNA for nucleosome assembly at the original location.
Title: The Mcm2-Ctf4-Polα Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands.
An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-alpha to Mcm2-7.
Title: An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-α to Mcm2-7.
Mcm10 directly binds the Minichromosome Maintenance Complex via Mcm2 and the Mcm6 C terminus.
Title: Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex.
Data show that Dbf4-dependent kinase (DDK)-phosphoryled MCM DNA helicase complex subunit MCM2 preferentially interacts with DNA replication initiation factor CDC45.
Title: Conserved mechanism for coordinating replication fork helicase assembly with phosphorylation of the helicase.
Mcm2-7 and specifically the ATPase site inactivated by mcm2DENQ are required at an intermediate step of the DNA replication checkpoint signal transduction cascade.
Title: Mcm2-7 Is an Active Player in the DNA Replication Checkpoint Signaling Cascade via Proposed Modulation of Its DNA Gate.
Mcm2-7 phosphorylation and firing factor recruitment promote complete and semi-conservative replication of the input plasmids.
Title: Prereplicative complexes assembled in vitro support origin-dependent and independent DNA replication.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA replication origin binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
contributes_to single-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to single-stranded DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to single-stranded DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA strand elongation involved in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA unwinding involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA unwinding involved in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via break-induced replication	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IEA Inferred from Electronic Annotation more info
involved_in premeiotic DNA replication	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of CMG complex	IDA Inferred from Direct Assay more info	PubMed
part_of CMG complex	IEA Inferred from Electronic Annotation more info
part_of DNA replication preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
part_of MCM complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MCM complex	IDA Inferred from Direct Assay more info	PubMed
part_of MCM complex	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of nuclear pre-replicative complex	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear pre-replicative complex	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed
part_of replication fork protection complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: MCM DNA helicase complex subunit MCM2

NP_009530.1

EC 3.6.4.12

Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; binds and transfers parental H3-H4 to Pol32p facilitating the transfer of parental histones to the lagging strand via Pol1p during DNA replication; relative distribution to the nucleus increases upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

174920..177526 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178263.1 → NP_009530.1 TPA: MCM DNA helicase complex subunit MCM2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009530.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VPX7, P29469

UniProtKB/TrEMBL

A6ZKS5, B3LNG0, C7GX87, C8Z3X3, G2W8Y6, N1PAX9

Conserved Domains (3) summary

smart00350
Location:302 → 846

MCM; minichromosome maintenance proteins

pfam12619
Location:49 → 193

MCM2_N; Mini-chromosome maintenance protein 2

pfam14551
Location:209 → 298

MCM_N; MCM N-terminal domain