Pcmt1 protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pcmt1provided by RGD
Official Full Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase 1provided by RGD
Primary source: RGD:3268
See related: EnsemblRapid:ENSRNOG00000014330 AllianceGenome:RGD:3268
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PCM
Summary: Enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity. Involved in several processes, including S-adenosylhomocysteine metabolic process; cellular response to hypoxia; and negative regulation of cardiac muscle cell apoptotic process. Located in several cellular components, including basolateral plasma membrane; brush border membrane; and perikaryon. Biomarker of high grade glioma. Orthologous to human PCMT1 (protein-L-isoaspartate (D-aspartate) O-methyltransferase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 550.2), Heart (RPKM 539.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1p13

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (3932161..3980751, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (2111756..2160354, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (1734863..1767759, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PIMT was critical for bFGF-mediated neuronal differentiation of PC12 cells and regulated the MAPK and Akt signaling pathways.
Title: Critical role of protein L-isoaspartyl methyltransferase in basic fibroblast growth factor-mediated neuronal cell differentiation.
ERK2-mediated phosphorylation of transcriptional coactivator binding protein PIMT/NCoA6IP at Ser298 augments hepatic gluconeogenesis.
Title: ERK2-mediated phosphorylation of transcriptional coactivator binding protein PIMT/NCoA6IP at Ser298 augments hepatic gluconeogenesis.
isoforms of the PIMT/Tgs1 protein with an RNA methyltransferase domain function both in the nucleus and in the cytoplasm
Title: Different isoforms of PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase localizes to the cytoplasm and nucleus.
PIMT-KO phenotype results from the cumulative effect of isoaspartate-related damage to a number of the neuron-rich proteins detected in this study
Title: Protein repair in the brain, proteomic analysis of endogenous substrates for protein L-isoaspartyl methyltransferase in mouse brain.
Ethanol consumption results in the accumulation of modified proteins bearing atypical isoaspartyl residues via impaired in vivo PIMT activity, while betaine administration prevents it by restoring the PIMT-catalyzed protein repair reaction
Title: Accumulation of proteins bearing atypical isoaspartyl residues in livers of alcohol-fed rats is prevented by betaine administration: effects on protein-L-isoaspartyl methyltransferase activity.

Submit: New GeneRIF Correction

General gene information

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Markers

RH130061 (e-PCR)
- UniSTS:213349

RH140246 (e-PCR)
- UniSTS:222325

RH130661 (e-PCR)
- UniSTS:213945

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables S-adenosylmethionine-dependent methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in S-adenosylhomocysteine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in S-adenosylmethionine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in methylation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cardiac muscle cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein methylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein methylation	ISO Inferred from Sequence Orthology more info
involved_in protein methylation	ISO Inferred from Sequence Orthology more info
involved_in protein modification process	IEA Inferred from Electronic Annotation more info
involved_in response to ethanol	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in brush border membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in perikaryon	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Names: L-isoaspartyl protein carboxyl methyltransferase; PIMT; protein L-isoaspartyl/D-aspartyl methyltransferase; protein-beta-aspartate methyltransferase

NP_001385490.1

EC 2.1.1.77

NP_001385491.1

EC 2.1.1.77

NP_037205.3

EC 2.1.1.77

XP_038957965.1

EC 2.1.1.77

XP_038957986.1

EC 2.1.1.77

XP_063138121.1

EC 2.1.1.77

XP_063138126.1

EC 2.1.1.77

XP_063138131.1

EC 2.1.1.77

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001398561.1 → NP_001385490.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform a

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/TrEMBL

A6JP10

Conserved Domains (1) summary

pfam01135
Location:66 → 274

PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
NM_001398562.1 → NP_001385491.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform c

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

Related

ENSRNOP00000108494.1, ENSRNOT00000150113.1

Conserved Domains (1) summary

pfam01135
Location:66 → 226

PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
NM_013073.4 → NP_037205.3 protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform b

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/TrEMBL

A0A140TAB9, A6JP12

Related

ENSRNOP00000019623.8, ENSRNOT00000019623.9

Conserved Domains (1) summary

pfam01135
Location:66 → 274

PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

3932161..3980751 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039102058.2 → XP_038957986.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform X3

UniProtKB/TrEMBL

A6JP11, A6JP13

Conserved Domains (1) summary

pfam01135
Location:1 → 185

PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
XM_063282051.1 → XP_063138121.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform X2
XM_039102037.2 → XP_038957965.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform X1

UniProtKB/TrEMBL

A6JP10

Related

ENSRNOP00000103701.1, ENSRNOT00000123595.1

Conserved Domains (1) summary

pfam01135
Location:77 → 239

PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
XM_063282056.1 → XP_063138126.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform X4
XM_063282061.1 → XP_063138131.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform X5

UniProtKB/TrEMBL

A6JP13