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    PRKCI protein kinase C iota [ Homo sapiens (human) ]

    Gene ID: 5584, updated on 3-Dec-2024

    Summary

    Official Symbol
    PRKCIprovided by HGNC
    Official Full Name
    protein kinase C iotaprovided by HGNC
    Primary source
    HGNC:HGNC:9404
    See related
    Ensembl:ENSG00000163558 MIM:600539; AllianceGenome:HGNC:9404
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    PKCI; DXS1179E; nPKC-iota
    Summary
    This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in stomach (RPKM 19.1), thyroid (RPKM 17.9) and 24 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See PRKCI in Genome Data Viewer
    Location:
    3q26.2
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 3 NC_000003.12 (170222424..170305977)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 3 NC_060927.1 (173007078..173090623)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (169940212..170023765)

    Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene polyhomeotic homolog 3 Neighboring gene RNA, U6 small nuclear 315, pseudogene Neighboring gene RNA, U7 small nuclear 32 pseudogene Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14880 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14881 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20799 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20800 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20801 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14882 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14883 Neighboring gene Sharpr-MPRA regulatory region 5121 Neighboring gene MPRA-validated peak4916 silencer Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20802 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20803 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14884 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14885 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14886 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14887 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:170079595-170080095 Neighboring gene MPRA-validated peak4917 silencer Neighboring gene SKI like proto-oncogene Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:170136404-170137356 Neighboring gene microRNA 6828 Neighboring gene claudin 11

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env HIV-1 gp120 upregulates the expression of protein kinase C, iota (PRKCI) in human B cells PubMed
    env HIV-1 gp120 activates forward trafficking and surface clustering of NMDA receptors in membrane micro domains by a PKA-dependent phosphorylation of the NR1 C-terminal Ser897, followed by a PKC-dependent phosphorylation of Ser896 PubMed
    env Pre-treatment of endothelial cells with fibroblast growth factor 2 (FGF2) protects cells from HIV-1 gp120 angiotoxicity; this protection is regulated by crosstalk among the ERK, PI3K-AKT and PKC signaling pathways PubMed
    env Down modulation of the interaction between HIV-1 gp120 and CD4 by TPA is blocked by protein kinase C (PKC) inhibitors, suggesting PKC may play an important role in HIV-1 infection PubMed
    env IL-16 induces rapid translocation of PKC from the cytosol to the membrane in CD4+ cells; PKC inhibitors completely block IL-16-induced lymphocyte migration as well as the motile response induced by HIV-1 gp120 and anti-CD4 antibody binding to CD4 PubMed
    env c-FLIPL inhibits Bax activation via modulating PKC expression at the transcriptional level involving AP-2 during gp120 treatment PubMed
    env A specific interaction between CD4 and HIV-1 gp120 is required for phosphorylation of CD4, which could involve protein kinase C PubMed
    env HIV-1 gp120 increases the levels of calcium-dependent and -independent PKC isozymes; the most striking change is observed in PKC-zeta isozyme levels PubMed
    env Induction of apoptosis in cell cultures through binding of HIV-1 gp120 or gp160 to CXCR4 involves protein kinase C, basic fibroblast growth factor, caspase-3, and the pro-apoptotic molecule Bax PubMed
    Envelope surface glycoprotein gp160, precursor env HIV-1 gp160-induced AP-1 complex formation is dependent upon protein tyrosine phosphorylation and is abolished by inhibitors of protein kinase C, but it is unaffected by calcium channel blockers or cyclosporine A PubMed
    Envelope transmembrane glycoprotein gp41 env A synthetic peptide corresponding to cytoplasmic domain residues 828-848 of HIV-1 gp41 inhibits pKC-catalysed phosphorylation of a protein substrate PubMed
    env A synthetic peptide containing residues 581-597 from HIV-1 gp41 inhibits protein kinase C (pkC)-mediated phosphorylation of the CD3 gamma-chain in intact cells and directly inhibits partially purified pkC PubMed
    Pr55(Gag) gag The phosphorylation of HIV-1 Gag by PRKCI regulates HIV-1 Vpr incorporation into virions and HIV-1 replication in macrophages PubMed
    gag Immunoprecipation analysis demonstrates that PRKCI binds HIV-1 Gag and phosphorylates Gag at position serine 487 in the p6 domain of Gag PubMed
    gag The amplified luminescent proximity homogeneous assay (AlphaScreen) identifies the interaction of HIV-1 Gag with PRKCI PubMed
    Tat tat Phospholipase C/protein kinase C signaling pathway-dependent phosphorylation of p44/42 and JNK MAP kinases participates partially in IL-1beta induction by TAT PubMed
    tat Protein kinase C is required for HIV-1 Tat-mediated transactivation of the viral LTR promoter, indicating protein kinase C regulates the process of HIV-1 transactivation and may play a role in the transition of HIV from latency to productive growth PubMed
    tat Protein kinase C phosphorylates HIV-1 Tat on serine residue 46 PubMed
    tat HIV-1 Tat activates protein kinase C, resulting in the induction of TNF-alpha, IL-6 and IL-10 expression and the secretion of MCP-1 PubMed
    Vpr vpr The phosphorylation of HIV-1 Gag at Ser487 by PRKCI regulates HIV-1 Vpr incorporation into virions and HIV-1 replication in macrophages PubMed
    matrix gag Protein kinase C (PKC) phosphorylates HIV-1 Matrix on serine residue 111 resulting in a shift in localization of Matrix from the cytosol to the cellular membrane, suggesting a myristoyl-protein switch regulated by PKC phosphorylation PubMed
    retropepsin gag-pol Phosphorylation of human recombinant vimentin by PKC inhibits the proteolytic processing of the vimentin head domain by HIV-1 protease PubMed
    reverse transcriptase gag-pol HIV-1 RT heterodimer expressed in bacteria can be phosphorylated in vitro by several purified mammalian protein kinases including auto-activated protein kinase (PK), CKII, cytosolic protamine kinase (CPK), myelin basic protein kinase 1 (MBPK1), and PRKC PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • MGC26534

    Gene Ontology Provided by GOA

    Process Evidence Code Pubs
    involved_in Golgi vesicle budding IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in actin filament organization IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cell migration IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cell-cell junction organization IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in cellular response to chemical stress TAS
    Traceable Author Statement
    more info
     
    involved_in cellular response to insulin stimulus IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in cellular response to insulin stimulus ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in cytoskeleton organization NAS
    Non-traceable Author Statement
    more info
    PubMed 
    involved_in establishment of apical/basal cell polarity IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in establishment of cell polarity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in establishment or maintenance of epithelial cell apical/basal polarity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in establishment or maintenance of epithelial cell apical/basal polarity ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in establishment or maintenance of epithelial cell apical/basal polarity TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in eye photoreceptor cell development IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in intracellular signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in membrane organization NAS
    Non-traceable Author Statement
    more info
    PubMed 
    involved_in negative regulation of apoptotic process TAS
    Traceable Author Statement
    more info
     
    involved_in negative regulation of glial cell apoptotic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in negative regulation of neuron apoptotic process IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of D-glucose import ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in positive regulation of NF-kappaB transcription factor activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of Notch signaling pathway TAS
    Traceable Author Statement
    more info
     
    involved_in positive regulation of endothelial cell apoptotic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of glial cell proliferation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of neuron projection development IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of neuron projection development IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of protein localization to plasma membrane ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in protein localization to plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in protein targeting to membrane NAS
    Non-traceable Author Statement
    more info
    PubMed 
    involved_in regulation of postsynaptic membrane neurotransmitter receptor levels IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in response to interleukin-1 IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in secretion NAS
    Non-traceable Author Statement
    more info
    PubMed 
    involved_in vesicle-mediated transport TAS
    Traceable Author Statement
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in Golgi membrane IEA
    Inferred from Electronic Annotation
    more info
     
    part_of PAR polarity complex IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    part_of PAR polarity complex ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in Schaffer collateral - CA1 synapse IEA
    Inferred from Electronic Annotation
    more info
     
    located_in Schmidt-Lanterman incisure IEA
    Inferred from Electronic Annotation
    more info
     
    located_in apical plasma membrane IEA
    Inferred from Electronic Annotation
    more info
     
    located_in bicellular tight junction IEA
    Inferred from Electronic Annotation
    more info
     
    located_in brush border IEA
    Inferred from Electronic Annotation
    more info
     
    located_in cell leading edge IEA
    Inferred from Electronic Annotation
    more info
     
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in endosome IEA
    Inferred from Electronic Annotation
    more info
     
    located_in extracellular exosome HDA PubMed 
    located_in glutamatergic synapse IEA
    Inferred from Electronic Annotation
    more info
     
    located_in intercellular bridge IDA
    Inferred from Direct Assay
    more info
     
    located_in microtubule cytoskeleton IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
     
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in plasma membrane TAS
    Traceable Author Statement
    more info
     
    located_in tight junction NAS
    Non-traceable Author Statement
    more info
    PubMed 

    General protein information

    Preferred Names
    protein kinase C iota type
    Names
    PRKC-lambda/iota
    aPKC-lambda/iota
    atypical protein kinase C-lambda/iota
    NP_002731.4
    XP_047304530.1
    XP_047304531.1
    XP_054203228.1
    XP_054203229.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_002740.6NP_002731.4  protein kinase C iota type

      See identical proteins and their annotated locations for NP_002731.4

      Status: REVIEWED

      Source sequence(s)
      AC023891, AC073288, BC022016, CN412350, L33881
      Consensus CDS
      CCDS3212.2
      UniProtKB/Swiss-Prot
      D3DNQ4, P41743, Q8WW06
      UniProtKB/TrEMBL
      B5BUK7
      Related
      ENSP00000295797.4, ENST00000295797.5
      Conserved Domains (3) summary
      cd06404
      Location:26108
      PB1_aPKC; PB1 domain is an essential modular domain of the atypical protein kinase C (aPKC) which in complex with Par6 and Par3 proteins is crucial for establishment of apical-basal polarity of animal cells. PB1 domain is a modular domain mediating specific ...
      cd05618
      Location:233596
      STKc_aPKC_iota; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota
      pfam00130
      Location:141191
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000003.12 Reference GRCh38.p14 Primary Assembly

      Range
      170222424..170305977
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047448574.1XP_047304530.1  protein kinase C iota type isoform X1

    2. XM_047448575.1XP_047304531.1  protein kinase C iota type isoform X2

      UniProtKB/TrEMBL
      B5BUK7

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060927.1 Alternate T2T-CHM13v2.0

      Range
      173007078..173090623
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054347253.1XP_054203228.1  protein kinase C iota type isoform X1

    2. XM_054347254.1XP_054203229.1  protein kinase C iota type isoform X2

      UniProtKB/TrEMBL
      B5BUK7