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Platform GPL11117 Query DataSets for GPL11117
Status Public on Oct 27, 2010
Title Brucella 16M whole genome microarray
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Brucella melitensis bv. 1 str. 16M
Manufacturer Beijing Institute of Disease Control and Prevention, P. R.China.
Manufacture protocol 1.Primers specifc for each of the ORFs of 16M genome sequence are designed by Arraydesigner 3.0. Primers are synthesized in 96-well plates.
2. Products of each of the ORFs were amplified in 96-well plate.
3. PCR products are purified in 96-well plates.
4. PCR products are disolved in 50% DMSO, gyrB, glk and 16s rRNA are included in each of the blocks as positive contrl, and 50% DMSO as negative control. These products are transferred into 384-well plates.
5. Products are printed in duplicate on VAL S25 glass slides (ECL); Each slide is composed of 32 sub-arrays in 18 columns and 12 rows.
6. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.
 
 
Submission date Oct 26, 2010
Last update date Oct 27, 2010
Contact name Zeliang Chen
E-mail(s) [email protected]
Organization name Beijing Institute of Disease Control and Prevention
Street address NO. 20,Dongdajie
City Beijing
ZIP/Postal code 100071
Country China
 
Samples (8) GSM613075, GSM613076, GSM613077, GSM613078, GSM1129940, GSM1129941 
Series (2)
GSE24942 Comparative transcriptome analysis of 16M and its vjbR mutant under a condition simulating environments of macrophage
GSE46418 Comparative transcriptome analysis of Brucella melitensis 16M and its hfq mutant under acidified minimum medium (GEM4.0) conditions

Data table header descriptions
ID
ORF Gene ID of Brucella 16M
PT_ACC genebank Protein accession number
gene symbol Gene symbol
description product name

Data table
ID ORF PT_ACC gene symbol description
BMEI0001 BMEI0001 NP_538919.1 hemE uroporphyrinogen decarboxylase
BMEI0002 BMEI0002 NP_538920.1 - cytochrome functioning/assembly related protein
BMEI0003 BMEI0003 NP_538921.1 rho transcription termination factor Rho
BMEI0004 BMEI0004 NP_538922.1 - putative cytoplasmic protein
BMEI0005 BMEI0005 NP_538923.1 - thymidylate kinase
BMEI0006 BMEI0006 NP_538924.1 trmE tRNA modification GTPase TrmE
BMEI0007 BMEI0007 NP_538925.1 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA
BMEI0008 BMEI0008 NP_538926.1 gidB 16S rRNA methyltransferase GidB
BMEI0009 BMEI0009 NP_538927.1 - chromosome partitioning protein PARA
BMEI0010 BMEI0010 NP_538928.1 - chromosome partitioning protein PARB
BMEI0011 BMEI0011 NP_538929.1 holA DNA polymerase III subunit delta
BMEI0012 BMEI0012 NP_538930.1 - ABC transporter ATP-binding protein
BMEI0013 BMEI0013 NP_538931.1 - ABC transporter membrane protein
BMEI0014 BMEI0014 NP_538932.1 - hypothetical protein BMEI0014
BMEI0015 BMEI0015 NP_538933.1 - ABC transporter substrate-binding protein
BMEI0016 BMEI0016 NP_538934.1 - hypothetical protein BMEI0016
BMEI0017 BMEI0017 NP_538935.1 - alkanal monooxygenase subunit alpha
BMEI0018 BMEI0018 NP_538936.1 - putative cytoplasmic protein
BMEI0019 BMEI0019 NP_538937.1 - LACI-family transcription regulator
BMEI0020 BMEI0020 NP_538938.1 - glucose-fructose oxidoreductase precursor

Total number of rows: 3192

Table truncated, full table size 206 Kbytes.




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