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Status
Public on Aug 04, 2004
Title
DKFZ Operon 27k long oligonucleotide array
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Homo sapiens
Description
Spotted long oligonucleotide array produced using the Operon Human Genome Oligo Set Version 2.1 (21,329 70mer oligonucleotides, based on build 147 of the Human UniGene database and the Human RefSeq Database, plus 24 controls) and the Operon Human Genome Oligo Set Version 2.1 Upgrade (5,462 70mer oligonucleotides, based on build 13.31 of the Human Ensembl Database). Spotting was performed in duplicates (replicate arrays) with 4x6 SMP3 pins (Telechem) at 18-20 °C and 40-50% RH on epoxysilane coated slides (Quantifoil) using 40 µM oligos in FBNC spotting buffer (G. Wrobel et al., Nucleic Acids Res 31 e67, 2003). Spot centers were 129 µm apart. DNA adhesion was accomplished by 1 h incubation at 60 °C followed by 240 mJ/cm2 UV irradiation at 254 nm.
Web link
http://www.dkfz-heidelberg.de/kompl_genome/index.html Contributor(s)
Schlingemann J , Thuerigen O , Toedt G , Kramer H , Hahn M , Lichter P Citation(s)
15718295 Submission date
Jul 28, 2004
Last update date
Apr 09, 2008
Contact name
Joerg Schlingemann
E-mail(s)
[email protected] Phone
+49 6221 424672
Fax
+49 6221 424639
URL
http://www.dkfz-heidelberg.de/kompl_genome/index.html Organization name
German Cancer Research Center (DKFZ)
Department
Molecular Biology (B060)
Lab
Expression Profiling Facility
Street address
Technologiepark III, Im Neuenheimer Feld 280
City
Heidelberg
ZIP/Postal code
69120
Country
Germany
Samples (32)
GSM27816 , GSM27817 , GSM27818 , GSM27819 , GSM27835 , GSM27836
GSM27915 ,
GSM27916 ,
GSM27917 ,
GSM27918 ,
GSM27919 ,
GSM27920 ,
GSM27921 ,
GSM27922 ,
GSM27923 ,
GSM27924 ,
GSM27925 ,
GSM27926 ,
GSM27927 ,
GSM27928 ,
GSM29755 ,
GSM29756 ,
GSM29757 ,
GSM29758 ,
GSM29808 ,
GSM29809 ,
GSM29810 ,
GSM29811 ,
GSM29812 ,
GSM29813 ,
GSM29818 ,
GSM29820 Series (2)
GSE1645 TAcKLE - Transcriptome Amplification and cDNA Labeling for Expression analysis
GSE1722 Cross-platform reproducibility of oligonucleotide microarray expression profiles
Data table header descriptions ID GB_ACC NCBI GenBank accession number or NCBI RefSeq accession number;
GB_ACC NCBI GenBank accession number or NCBI RefSeq accession number;
CLONE ID Internal DKFZ database ID (CloneY)
GENE_NAME Gene name
GENE_SYMBOL Gene symbol
ENSEMBL_GENE_ID Ensembl identifier (gene ID)
GENE Entrez GENE ID
PLATE spotting plate no.
ROW spotting row no.
COLUMN spotting column no.
SPOT_ID
Data table ID GB_ACC GB_ACC CLONE ID GENE_NAME GENE_SYMBOL ENSEMBL_GENE_ID GENE PLATE ROW COLUMN SPOT_ID 1
OLC00001
control oligo
1
1
1
control oligo
2
OLC00001
control oligo
1
1
2
control oligo
3
X14672 NM_000015 OL000001
ARYLAMINE N-ACETYLTRANSFERASE 2
NAT2
ENSG00000156006
10 1
1
3
4
Z32564 NM_000804 OL000096
FOLATE RECEPTOR GAMMA PRECURSOR
FOLR3
ENSG00000110203
2352 1
1
4
5
M12271 NM_000667 OL000002
ALCOHOL DEHYDROGENASE CLASS III CHI CHAIN
ADH1C
ENSG00000164025
126 1
1
5
6
AC003682 NM_003435 OL000097
ZINC FINGER PROTEIN 134
ZNF134
ENSG00000166680
7693 1
1
6
7
L00692 NM_001815 OL000003
CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 3 PRECURSOR
CEACAM3
ENSG00000170956
1084 1
1
7
8
D25234 NM_000317 OL000098
6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE
PTS
ENSG00000150787
5805 1
1
8
9
D90276 NM_001817 OL000004
CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 4, CARCINOEMBRYONIC ANTIGEN GENE FAMILY MEMB
CEACAM4
ENSG00000105352
1089 1
1
9
10
OL000099
1
1
10
OL000099
11
M98447 NM_000359 OL000005
PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE K
TGM1
ENSG00000092295
7051 1
1
11
12
U07882 NM_000911 OL000100
DELTA-TYPE OPIOID RECEPTOR
OPRD1
ENSG00000116329
4985 1
1
12
13
D00726 NM_000140 OL000006
FERROCHELATASE, MITOCHONDRIAL PRECURSOR
FECH
ENSG00000066926
2235 1
1
13
14
U03505 NM_004171 OL000101
EXCITATORY AMINO ACID TRANSPORTER 2
SLC1A2
ENSG00000110436
6506 1
1
14
15
M63635 NM_000170 OL000007
GLYCINE DEHYDROGENASE [DECARBOXYLATING], MITOCHONDRIAL PRECURSOR
GLDC
ENSG00000049496
2731 1
1
15
16
U02687 NM_004119 OL000102
FL CYTOKINE RECEPTOR PRECURSOR
FLT3
ENSG00000122025
2322 1
1
16
17
D10583 NM_000139 OL000008
HIGH AFFINITY IMMUNOGLOBULIN EPSILON RECEPTOR BETA-SUBUNIT
MS4A2
ENSG00000149534
2206 1
1
17
18
D16581 NM_002452 OL000103
7,8-DIHYDRO-8-OXOGUANINE TRIPHOSPHATASE
NUDT1
ENSG00000106268
4521 1
1
18
19
D11327 NM_080589 OL000009
PROTEIN-TYROSINE PHOSPHATASE, NON-RECEPTOR TYPE 7
PTPN7
ENSG00000143851
5778 1
1
19
20
M12271 NM_000667 OL000104
ALCOHOL DEHYDROGENASE CLASS III CHI CHAIN
ADH1C
ENSG00000164025
126 1
1
20
27648 1991 Kbytes .Supplementary data files not provided
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