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Platform GPL15698 Query DataSets for GPL15698
Status Public on Dec 31, 2014
Title CIMAP Artemisia annua (cv. CIM-Arogya) microarray platform
Technology type spotted oligonucleotide
Distribution custom-commercial
Organism Artemisia annua
Manufacturer Ocimum Biosolutions Ltd., Hyderabad, India
Manufacture protocol Five microgram of total RNA was used for amplification using Amino Allyl MessageAmp™ II aRNA Amplification Kit from Ambion by linear transcription based RNA amplification system to produce cRNA. Briefly, mRNA was reverse transcribed with a oligo (dT) primer bearing T7 promoter at 42°C for 2 hrs and second strand cDNA synthesis was carried out at 16°C for 2 hrs. The resulting cDNA was then purified and was then transcribed with T7 RNA polymerase to generate multiple copies of aminoallyl antisense RNA (aRNA) at 37°C for 16 hrs. aRNA was then labelled with Cy3TM/Cy5TM post-labelling reactive dye pack (GE Healthcare, UK) at room temperature and unincorporated Cy3/Cy5 molecules were removed by purification process. Samples were purified using QIAGEN PCR purification kit before hybridization.
 
 
Contributor(s) Nair P, Misra A, Shukla AK, Shasany AK, Khanuja S
Submission date Jun 15, 2012
Last update date Dec 31, 2014
Contact name Ajit K. Shasany
E-mail(s) [email protected]
Phone +91-522-2718548
Organization name CSIR-Central Institute of Medicinal and Aromatic Plants
Department Biotechnology Division
Street address P.O. CIMAP
City Lucknow
State/province Uttar Pradesh
ZIP/Postal code 226015
Country India
 
Samples (2) GSM956130, GSM956131
Series (1)
GSE39098 Comparative gene expression analysis in Artemisia annua (cv. CIM-Arogya) seedling and mature leaf tissues having contrasting artemisinin content

Data table header descriptions
ID
SEQUENCE Probe Sequence
UniGene_Sequence_Accession Unigenes and Expressed Sequence Tags, sequences that are expressed (RNA) but have not been shown to code for proteins
GI GI
GB_ACC GenBank Accession number
Unigene_ID Artemisia annua UniGene ID Number from NCBI

Data table
ID SEQUENCE UniGene_Sequence_Accession GI GB_ACC Unigene_ID
OBSca028_0001 CTGACACTCACATGAATTGGAGGACAACCAGTCGAACACCCATATATCAC gi|159596583|gb|EY109430|gnl|UG|Aan_S41967016 CAZI6267.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI6267 5 159596583 EY109430 Aan.17584
OBSca028_0002 ATTTGAGTCTCATTACCAAAGCTCGTCACAATGGGCAGAACTATGTCTTT gi|159676665|gb|EY104676|gnl|UG|Aan_S41962262 CAZI3856.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI3856 5 159676665 EY104676 Aan.7069
OBSca028_0003 CCCAACACACCGATAGTTACTATAGCGTAAATTATACCGAGGCTTCCAAA gi|159724450|gb|EY070784|gnl|UG|Aan_S41928370 CATG3342.fwd CATG Artemisia annua 159724450 EY070784 Aan.4782
OBSca028_0005 CGAGTAAACATCTCACCCTCATCATTAGGACCATCAACCACCTCAATTTC gi|159743976|gb|EY116413|gnl|UG|Aan_S41973999 CAZI987.rev CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI987 3 159743976 EY116413 Aan.3769
OBSca028_0006 GATGATGATGTGAAGTTGGCTAACAGCGTTGATGTTGCATCTCTTCGTGA gi|159598409|gb|EY080280|gnl|UG|Aan_S41937866 CAZI14232.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI14232 5 159598409 EY080280 Aan.13585
OBSca028_0007 AGAGGCAAGGGCAAGAACATCTTTACTGGCAGACATGCTGAGAACAAATT gi|159598408|gb|EY080279|gnl|UG|Aan_S41937865 CAZI14232.rev CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI14232 3 159598408 EY080279 Aan.13584
OBSca028_0008 ACTAGACTTACATCCTGCTATTCGTTCTTTGATCGGAAGTACCGTTGGAA gi|159781009|gb|EY090666|gnl|UG|Aan_S41948252 CAZI19495.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI19495 5 159781009 EY090666 Aan.13944
OBSca028_0009 GGATGCAGTGAGAGTTAAAGACCCAGAGTGGCTTATAGTTGTTGGAGTAT gi|159693017|gb|EY112148|gnl|UG|Aan_S41969734 CAZI7695.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI7695 5 159693017 EY112148 Aan.12246
OBSca028_0010 GTTTGATTTCATATCGTGACTTGGTGGGTGTGGCATATACAGAAGAGGAA gi|159711155|gb|EY088891|gnl|UG|Aan_S41946477 CAZI18611.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI18611 5 159711155 EY088891 Aan.11743
OBSca028_0011 GACGGATTAGTGATGCGTAAACGAACCTTCCACCAGTCAACACAAAGTAT gi|159693016|gb|EY112147|gnl|UG|Aan_S41969733 CAZI7695.rev CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI7695 3 159693016 EY112147 Aan.2505
OBSca028_0012 AGTCTGGAACAACTCCTGGATACTCTTACTCTGCTGCTAACAAGAACAAG gi|159639444|gb|EY060655|gnl|UG|Aan_S41918241 CATF12315.fwd CATF Artemisia annua 159639444 EY060655 Aan.8227
OBSca028_0013 CAACTAATTACCCAGGTCGTTTCCGTGTGTTCTATGTATTGAATCAGCCA gi|159666700|gb|EY096112|gnl|UG|Aan_S41953698 CAZI22563.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI22563 5 159666700 EY096112 Aan.6504
OBSca028_0014 TTGTCATAGGCCCTGTTCCTGGTAAGAAATATAGTGAAATCACCTTTCCT gi|159520899|gb|EY047794|gnl|UG|Aan_S41905380 CATB1094.fwd CATB Artemisia annua 159520899 EY047794 Aan.1045
OBSca028_0015 ACAGCCATCTTATTCTTCGGATGCAGGAATCGCAAAGTGGATTTCATATA gi|124020686|gb|EF197890|gnl|UG|Aan_S41974265 Artemisia annua cytochrome P450 reductase mRNA 124020686 EF197890 Aan.3134
OBSca028_0016 ATACACTCCTAATATGAAGAAACACATTGGCATGATTGCAGGTGGGTCAG gi|159721972|gb|EY109217|gnl|UG|Aan_S41966803 CAZI6149.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI6149 5 159721972 EY109217 Aan.11886
OBSca028_0017 GCCGCATAGCTCAATATCATCATACTTACGTGCATTATTAACCATTCCGA gi|159519945|gb|EY047399|gnl|UG|Aan_S41904985 CATA616.fwd CATA Artemisia annua 159519945 EY047399 Aan.4163
OBSca028_0018 GAAGACTTGTTCATATCTGTTTGGAATCTCATCGCTCTCCTACCCATTGG gi|159772678|gb|EY109770|gnl|UG|Aan_S41967356 CAZI645.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI645 5 159772678 EY109770 Aan.9829
OBSca028_0019 TTTAGGTGAAGAAGTCCGTAACAAACTATTCGATGATGTGTCAGCCCTTT gi|159609737|gb|EY077593|gnl|UG|Aan_S41935179 CAZI12875.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI12875 5 159609737 EY077593 Aan.11132
OBSca028_0020 AAGAGGAAAAGGCTTAGTGTTGAGAGGTTATTTGGGATCAGTTGCATTCA gi|159610279|gb|EY102076|gnl|UG|Aan_S41959662 CAZI25586.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI25586 5 159610279 EY102076 Aan.3091
OBSca028_0021 CACCATCTCATCACTCATTTCAGTTGGAAACCCGCCGAAGAAGATAAAGT gi|86279651|gb|DQ363132|gnl|UG|Aan_S41974289 Artemisia annua putative steroid 23-alpha-hydroxylase cytochrome P450 mRNA 86279651 DQ363132 Aan.6573

Total number of rows: 750

Table truncated, full table size 161 Kbytes.




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