GEO help: Mouse over screen elements for information.
Status
Public on May 01, 2013
Title
SYBR green based Human miRNA PCR assay panel
Technology type
RT-PCR
Distribution
virtual
Organism
Homo sapiens Manufacturer
n/a
Manufacture protocol
n/a
Catalog number
n/a
Description
This is a SYBR green based qPCR method to achieve higher accuracy and sensitivity of small RNA detection. E. Coli polyA polymerase was employed to add multiple adenines at the end of every RNA molecules in the total RNA pool that lacks of a polyA tail. With retro-transcriptase and oligodT annealing, a universal tag was introduced to the 3’ end of cDNAs. Using this tag, quantitative PCR was performed with microRNA-specific forward primer and reverse universal primer mix.
Submission date
Feb 14, 2013
Last update date
May 01, 2013
Contact name
zining zhang
E-mail(s)
[email protected] Organization name
China Medical University
Department
1st affiliated hospital
Lab
Center for AIDS Research
Street address
No 155, Nanjingbei street, Heping District
City
Shenyang
State/province
Liaoning
ZIP/Postal code
110001
Country
China
Samples (50)
GSM1083373 , GSM1083374 , GSM1083375 , GSM1083376 , GSM1083377 , GSM1083378
GSM1083379 ,
GSM1083380 ,
GSM1083381 ,
GSM1083382 ,
GSM1083383 ,
GSM1083384 ,
GSM1083385 ,
GSM1083386 ,
GSM1083387 ,
GSM1083388 ,
GSM1083389 ,
GSM1083390 ,
GSM1083391 ,
GSM1083392 ,
GSM1083393 ,
GSM1083394 ,
GSM1083395 ,
GSM1083396 ,
GSM1083397 ,
GSM1083398 ,
GSM1083399 ,
GSM1083400 ,
GSM1083401 ,
GSM1083402 ,
GSM1083403 ,
GSM1083404 ,
GSM1083405 ,
GSM1083406 ,
GSM1083407 ,
GSM1083408 ,
GSM1083409 ,
GSM1083410 ,
GSM1083411 ,
GSM1083412 ,
GSM1083413 ,
GSM1083414 ,
GSM1083415 ,
GSM1083416 ,
GSM1083417 ,
GSM1083418 ,
GSM1083419 ,
GSM1083420 ,
GSM1083421 ,
GSM1083422 Series (1)
GSE44332 Real-time quantitative PCR analysis of microRNAs in human PBMC samples
Data table header descriptions ID miRNA_ID
347 5 Kbytes .Supplementary data files not provided
Less...
More...