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Status
Public on Mar 07, 2018
Title
Protein Microarray_Neisseria meningitidis [fHbp, NHBA and NadA]
Technology type
spotted peptide or protein
Distribution
non-commercial
Organism
Neisseria meningitidis Manufacturer
Molecular biology Lab, AIMB, GSK Vaccines
Manufacture protocol
Purified recombinant protein were diluted at final concentration of 0.5 mg/ul in 40% glycerol and transferred into 384-well microtiterplates. One droplet of 0.2 nL protein solution was deposited per spot on nitrocellulose-coated slides (FAST slides, Schleicher and Schuell) using the non-contact printer Marathon Spotter (Arrayjet, Edinburgh, UK). Printing was performed in a cabinet with controlled humidity and temperature (55-60% and 12 °C respectively), resulting in spots of 90-110 μm in diameter range. Each protein was printed in 8 or 16 replicates on each slide. Printed protein microarrays were kept at room temperature for 5 hours, dried under a stream of nitrogen and store a 4°C.
Submission date
May 12, 2017
Last update date
Mar 07, 2018
Contact name
Erika Bartolini
Organization name
GSK Vaccines
Department
AIMB
Lab
Molecular Biology
Street address
Via Fiorentina 1
City
Siena
State/province
Italy
ZIP/Postal code
53100
Country
Italy
Samples (37)
GSM2617129 , GSM2617130 , GSM2617131 , GSM2617132 , GSM2617133 , GSM2617134
GSM2617135 ,
GSM2617136 ,
GSM2617137 ,
GSM2617138 ,
GSM2617139 ,
GSM2617140 ,
GSM2617141 ,
GSM2617142 ,
GSM2617143 ,
GSM2617144 ,
GSM2617145 ,
GSM2617146 ,
GSM2617147 ,
GSM2617148 ,
GSM2617149 ,
GSM2617150 ,
GSM2617151 ,
GSM2617152 ,
GSM2617153 ,
GSM2617154 ,
GSM2617155 ,
GSM2617156 ,
GSM2617157 ,
GSM2617158 ,
GSM2617159 ,
GSM2617160 ,
GSM2617161 ,
GSM2617162 ,
GSM2617163 ,
GSM2617164 ,
GSM2617165 Series (2)
GSE98878 Epitope mapping of human Fabs directed against 4CmenB protein components by protein microarray
GSE98883 Epitope mapping of human antibodies directed against 4CmenB protein components
Data table header descriptions ID Name common protein name
GI number accession number of spotted protein
aminoacid region the amino acid region of proteins spotted on the protein array
PT_ACC protein accession
accession_string
Data table ID Name GI number aminoacid region PT_ACC accession_string [1]
NadA
610425912
10-51
AHW74788.1 AHW74788.1GI: 610425912
[101]
NadA
610425912
1-350
AHW74788.1 AHW74788.1GI: 610425912
[102]
NHBA
254547343
full length
ACT66702.1 gi|254547343|ref|ACT66702.1
[103]
fHbp var1
7227128
full length
AAF42204.1 AAF42204.1GI: 7227128
[104]
fHbp var2
7228704
full length
AAF42586.1 gi|7228704|ref|AAF42586.1
[105]
fHbp var3
106073479
full length
ABF82029.1 ABF82029.1GI: 106073479
[108]
NUbp
7226269
full length
AAF41429.1 gi|7226269|ref|AAF41429.1
[11]
NadA
610425912
74-143
AHW74788.1 AHW74788.1GI: 610425912
[111]
NHBA
254547343
full length
ACT66702.1 gi|254547343|ref|ACT66702.1
[12]
NadA
610425912
75-128
AHW74788.1 AHW74788.1GI: 610425912
[13]
NadA
610425912
97-130
AHW74788.1 AHW74788.1GI: 610425912
[15]
NadA
610425912
107-195
AHW74788.1 AHW74788.1GI: 610425912
[152]
fHbp var1
7227128
1-50
AAF42204.1 AAF42204.1GI: 7227128
[153]
fHbp var1
7227128
181-434
AAF42204.1 AAF42204.1GI: 7227128
[155]
fHbp var1
7227128
192-280
AAF42204.1 AAF42204.1GI: 7227128
[156]
fHbp var1
7227128
380-434
AAF42204.1 AAF42204.1GI: 7227128
[159]
NadA
610425912
100-275
AHW74788.1 AHW74788.1GI: 610425912
[160]
NadA
610425912
110-191
AHW74788.1 AHW74788.1GI: 610425912
[161]
NadA
610425912
158-350
AHW74788.1 AHW74788.1GI: 610425912
[162]
NadA
610425912
150-255
AHW74788.1 AHW74788.1GI: 610425912
102 6 Kbytes .Supplementary data files not provided
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