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Platform GPL2721 Query DataSets for GPL2721
Status Public on Aug 07, 2005
Title RIKILT Human Oligo Array
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Homo sapiens
Manufacturer Rikilt
Manufacture protocol The 10K MWG human oligoset together with additional 50-mer oligo probes involved in energy metabolism were in-house printed on GAP II coated slides (Corning), using the Microgrid II arrayer (BioRobotics Ltd, Cambridge, UK). After printing, microarrays were allowed to dry at room temperature. The oligo arrays were rehydrated and immobilized by UV cross-linking (120 MJ of UV energy). The microarray slides were then prewashed in the following order to reduce background; 20 minutes at 55C with 2x SSC/0.2% SDS, 5 minutes at room temperature (RT) with 0.2x SSC and 3 minutes at RT with demineralized water. Slides were dried by 2 minutes of centrifugation (2000 rpm).
Spotbuffer: 5* SSC
Concentration Oligo's: 700pmol/14 microliter
 
 
Submission date Aug 05, 2005
Last update date Mar 30, 2006
Contact name Jaap Keijer
Organization name RIKILT Institute of Food Safety
Street address Bornsesteeg 45
City Wageningen
ZIP/Postal code 6708 PD
Country Netherlands
 
Samples (22) GSM69207, GSM69208, GSM69209, GSM69210, GSM69211, GSM69212 
Series (1)
GSE3099 Folic Acid study in HT-29 cells

Data table header descriptions
ID
ArrayVisionID
block
row
column
GB_ACC GenBank Accession Number
MWG numbering
CDS-ID*
Accessionnumber
description
Unigene
useful accession Accession used for annotation
recplaced by Old accession numbers are replaced by these
UGCluster
Symbol
LLID All LocusLink ID's
LLID1
LLID2
LLID3
LLID4
LLID5
UniProt
Name
SPOT_ID spot identifier

Data table
ID ArrayVisionID block row column GB_ACC MWG numbering CDS-ID* Accessionnumber description Unigene useful accession recplaced by UGCluster Symbol LLID LLID1 LLID2 LLID3 LLID4 LLID5 UniProt Name SPOT_ID
R0 - R0 1 1 1 1 NM_005494 312 mwghuman30K#A:04664 NM_005494_1 similar to mrj gene for a member of the dnaj protein family (h. sapiens); dnajb6 NM_005494 490745 DNAJB6 10049 10049 O75190 DnaJ (Hsp40) homolog, subfamily B, member 6
R0 - R1 2 1 1 2 NM_014644 696 mwghuman30K#A:03293 NM_014644_1 myomegalin; pde4dip NM_014644 381235 PDE4DIP 9659 9659 O75042 Phosphodiesterase 4D interacting protein (myomegalin)
R0 - R2 3 1 1 3 NM_001675 1080 mwghuman30K#A:00770 NM_001675_1 activating transcription factor 4; atf4 NM_001675 496487 ATF4 468 468 P18848 Activating transcription factor 4 (tax-responsive enhancer element B67)
R0 - R3 4 1 1 4 NM_030984 1464 mwghuman30K#A:05234 NM_030984_1 thromboxane a synthase 1 (platelet, cytochrome p450, subfamily v), isoform txs-ii; tbxas1 NM_030984 520757 TBXAS1 6916; 389562 6916 389562 P24557 Thromboxane A synthase 1 (platelet, cytochrome P450, family 5, subfamily A)
R0 - R4 5 1 1 5 NM_024669 1848 mwghuman30K#A:05215 NM_024669_1 hypothetical protein flj11795; flj11795 NM_024669 436214 FLJ11795 79722 79722 Q9HAD3 Hypothetical protein FLJ11795
R0 - R5 6 1 1 6 NM_024012 2232 mwghuman30K#A:07724 NM_024012_1 5-hydroxytryptamine (serotonin) receptor 5a; htr5a NM_024012 65791 HTR5A 3361 3361 P47898 5-hydroxytryptamine (serotonin) receptor 5A
R0 - R6 7 1 1 7 NM_017757 2616 mwghuman30K#A:02813 NM_017757_1 hypothetical protein flj20307; flj20307 NM_017757 536490 ZNF407 55628 55628 Q9C0G0 Zinc finger protein 407
R0 - R7 8 1 1 8 NM_022341 3000 mwghuman30K#A:03315 NM_022341_1 peptide deformylase-like protein; loc64146 NM_022341 130849 COG8 84342; 64146 84342 64146 Q9HBH1 Component of oligomeric golgi complex 8
R0 - R8 9 1 1 9 NM_016529 3384 mwghuman30K#A:06199 NM_016529_1 atpase, aminophospholipid transporter-like, class i, type 8a, member 2; atp8a2 NM_016529 444957 ATP8A2 51761 51761 Q9NTI2 ATPase, aminophospholipid transporter-like, Class I, type 8A, member 2
R0 - R9 10 1 1 10 NM_021244 3768 mwghuman30K#A:01648 NM_021244_1 rag d protein; ragd NM_021244 485938 RRAGD 58528 58528 Q9NQL2 Ras-related GTP binding D
R0 - R10 11 1 1 11 NM_020216 4152 mwghuman30K#A:07775 NM_020216_1 arginyl aminopeptidase (aminopeptidase b); rnpep NM_020216 497391 RNPEP 6051 6051 Q9H4A4 Arginyl aminopeptidase (aminopeptidase B)
R0 - R11 12 1 1 12 NM_000355 4536 mwghuman30K#A:08934 NM_000355_1 transcobalamin ii; tcn2 NM_000355 417948 TCN2 6948 6948 P20062 Transcobalamin II; macrocytic anemia
R0 - R12 13 1 1 13 NM_017814 4920 mwghuman30K#A:06639 NM_017814_1 hypothetical protein flj20422; flj20422 NM_017814 26570 FLJ20422 54929 54929 Q9NX61 Hypothetical protein FLJ20422
R1 - C14 14 2 1 14 NM_006033 5304 mwghuman30K#A:01793 NM_006033_1 endothelial lipase precursor; lipg NM_006033 465102 LIPG 9388 9388 Q9Y5X9 Lipase, endothelial
R1 - C15 15 2 1 15 NM_002386 5688 mwghuman30K#A:01334 NM_002386_1 melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); mc1r NM_002386 513829 MC1R 4157 4157 Q01726 Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
R1 - C16 16 2 1 16 NM_017658 6072 mwghuman30K#A:03075 NM_017658_1 hypothetical protein flj20081; flj20081 NM_017658 174682 BTBD5 54813 54813 Q9NXS3 BTB (POZ) domain containing 5
R1 - C17 17 2 1 17 NM_001982 6456 mwghuman30K#A:06941 NM_001982_1 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); erbb3 NM_001982 118681 ERBB3 2065 2065 P21860 V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
R1 - C18 18 2 1 18 NM_002180 6840 mwghuman30K#A:07619 NM_002180_1 immunoglobulin mu binding protein 2; ighmbp2 NM_002180 503048 IGHMBP2 3508 3508 P38935 Immunoglobulin mu binding protein 2
R1 - C19 19 2 1 19 NM_014811 7224 mwghuman30K#A:08495 NM_014811_1 kiaa0649 gene product; kiaa0649 NM_014811 533260 KIAA0649 9858 9858 Q9Y4D3 KIAA0649
R1 - C20 20 2 1 20 NM_033015 7608 mwghuman30K#A:07077 NM_033015_1 fas-activated serine/threonine kinase, isoform 2; fastk NM_033015 75087 FASTK 10922 10922 Q14296 FAST kinase

Total number of rows: 10296

Table truncated, full table size 1968 Kbytes.






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