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Status
Public on Aug 28, 2020
Title
4CMenB antigenic components protein microarray
Technology type
spotted peptide or protein
Distribution
non-commercial
Organism
Neisseria meningitidis Manufacturer
Molecular biology Lab, AIMB, GSK Vaccines
Manufacture protocol
Purified recombinant protein were diluted at final concentration of 0.5 mg/ul in 40% glycerol and transferred into 384-well microtiterplates. One droplet of 0.2 nL protein solution was deposited per spot on nitrocellulose-coated slides (FAST slides, Schleicher and Schuell) using the non-contact printer Marathon Spotter (Arrayjet, Edinburgh, UK). Printing was performed in a cabinet with controlled humidity and temperature (55-60% and 12 °C respectively), resulting in spots of 90-110 μm in diameter range. Each protein was printed in 8 replicates on each slide. Printed protein microarrays were kept at room temperature for 5 hours, dried under a stream of nitrogen and store a 4°C.
Submission date
Jun 18, 2020
Last update date
Aug 27, 2020
Contact name
Erika Bartolini
Organization name
GSK Vaccines
Department
AIMB
Lab
Molecular Biology
Street address
Via Fiorentina 1
City
Siena
State/province
Italy
ZIP/Postal code
53100
Country
Italy
Samples (219)
GSM4626302 , GSM4626303 , GSM4626304 , GSM4626305 , GSM4626306 , GSM4626307
GSM4626308 ,
GSM4626309 ,
GSM4626310 ,
GSM4626311 ,
GSM4626312 ,
GSM4626313 ,
GSM4626314 ,
GSM4626315 ,
GSM4626316 ,
GSM4626317 ,
GSM4626318 ,
GSM4626319 ,
GSM4626320 ,
GSM4626321 ,
GSM4626322 ,
GSM4626323 ,
GSM4626324 ,
GSM4626325 ,
GSM4626326 ,
GSM4626327 ,
GSM4626328 ,
GSM4626329 ,
GSM4626330 ,
GSM4626331 ,
GSM4626332 ,
GSM4626333 ,
GSM4626334 ,
GSM4626335 ,
GSM4626336 ,
GSM4626337 ,
GSM4626338 ,
GSM4626339 ,
GSM4626340 ,
GSM4626341 ,
GSM4626342 ,
GSM4626343 ,
GSM4626344 ,
GSM4626345 ,
GSM4626346 ,
GSM4626347 ,
GSM4626348 ,
GSM4626349 ,
GSM4626350 ,
GSM4626351 ,
GSM4626352 ,
GSM4626353 ,
GSM4626354 ,
GSM4626355 ,
GSM4626356 ,
GSM4626357 ,
GSM4626358 ,
GSM4626359 ,
GSM4626360 ,
GSM4626361 ,
GSM4626362 ,
GSM4626363 ,
GSM4626364 ,
GSM4626365 ,
GSM4626366 ,
GSM4626367 ,
GSM4626368 ,
GSM4626369 ,
GSM4626370 ,
GSM4626371 ,
GSM4626372 ,
GSM4626373 ,
GSM4626374 ,
GSM4626375 ,
GSM4626376 ,
GSM4626377 ,
GSM4626378 ,
GSM4626379 ,
GSM4626380 ,
GSM4626381 ,
GSM4626382 ,
GSM4626383 ,
GSM4626384 ,
GSM4626385 ,
GSM4626386 ,
GSM4626387 ,
GSM4626388 ,
GSM4626389 ,
GSM4626390 ,
GSM4626391 ,
GSM4626392 ,
GSM4626393 ,
GSM4626394 ,
GSM4626395 ,
GSM4626396 ,
GSM4626397 ,
GSM4626398 ,
GSM4626399 ,
GSM4626400 ,
GSM4626401 ,
GSM4626402 ,
GSM4626403 ,
GSM4626404 ,
GSM4626405 ,
GSM4626406 ,
GSM4626407 ,
GSM4626408 ,
GSM4626409 ,
GSM4626410 ,
GSM4626411 ,
GSM4626412 ,
GSM4626413 ,
GSM4626414 ,
GSM4626415 ,
GSM4626416 ,
GSM4626417 ,
GSM4626418 ,
GSM4626419 ,
GSM4626420 ,
GSM4626421 ,
GSM4626422 ,
GSM4626423 ,
GSM4626424 ,
GSM4626425 ,
GSM4626426 ,
GSM4626427 ,
GSM4626428 ,
GSM4626429 ,
GSM4626430 ,
GSM4626431 ,
GSM4626432 ,
GSM4626433 ,
GSM4626434 ,
GSM4626435 ,
GSM4626436 ,
GSM4626437 ,
GSM4626438 ,
GSM4626439 ,
GSM4626440 ,
GSM4626441 ,
GSM4626442 ,
GSM4626443 ,
GSM4626444 ,
GSM4626445 ,
GSM4626446 ,
GSM4626447 ,
GSM4626448 ,
GSM4626449 ,
GSM4626450 ,
GSM4626451 ,
GSM4626452 ,
GSM4626453 ,
GSM4626454 ,
GSM4626455 ,
GSM4626456 ,
GSM4626457 ,
GSM4626458 ,
GSM4626459 ,
GSM4626460 ,
GSM4626461 ,
GSM4626462 ,
GSM4626463 ,
GSM4626464 ,
GSM4626465 ,
GSM4626466 ,
GSM4626467 ,
GSM4626468 ,
GSM4626469 ,
GSM4626470 ,
GSM4626471 ,
GSM4626472 ,
GSM4626473 ,
GSM4626474 ,
GSM4626475 ,
GSM4626476 ,
GSM4626477 ,
GSM4626478 ,
GSM4626479 ,
GSM4626480 ,
GSM4626481 ,
GSM4626482 ,
GSM4626484 ,
GSM4626486 ,
GSM4626489 ,
GSM4626491 ,
GSM4626493 ,
GSM4626495 ,
GSM4626497 ,
GSM4626499 ,
GSM4626502 ,
GSM4626504 ,
GSM4626506 ,
GSM4626508 ,
GSM4626510 ,
GSM4626512 ,
GSM4626514 ,
GSM4626516 ,
GSM4626517 ,
GSM4626518 ,
GSM4626519 ,
GSM4626520 ,
GSM4626521 ,
GSM4626522 ,
GSM4626523 ,
GSM4626524 ,
GSM4626525 ,
GSM4626526 ,
GSM4626527 ,
GSM4626528 ,
GSM4626529 ,
GSM4626530 ,
GSM4626531 ,
GSM4626532 ,
GSM4626533 ,
GSM4626534 ,
GSM4626535 ,
GSM4626536 ,
GSM4626537 ,
GSM4626538 Series (1)
GSE152785 Immunological fingerprint of 4CMenB recombinant antigens via protein microarray
Data table header descriptions ID Name common protein name
aminoacid region the aminoacid region of NHBA, fHbp and NadA proteins spotted on the protein array
GI number accession number
PT_ACC PT_GI protein tag manuscript code code used in the manuscript for fragment identification
Data table ID Name aminoacid region GI number PT_ACC PT_GI protein tag manuscript code [110]
936 (GNA2091)
full length
AAF42408.1 GI:7227353
AAF42408.1
7227353
Tagless
A-01
[190]
936 (GNA2091)
full length
AAF42408.1 GI:7227354
AAF42408.1
7227354
Trx-His
A-02
[217]
936 (GNA2091)
full length
AAF42408.1 GI:7227355
AAF42408.1
7227355
Tagless
A-03
[191]
936 (GNA2091)
1-180
AAF42408.1 GI:7227356
AAF42408.1
7227356
Trx-His
A-04
[154]
936 (GNA2091)
1-91
AAF42408.1 GI:7227357
AAF42408.1
7227357
Trx-His
A-05
[152]
fHbp var1
1-50
AAF42204.1GI: 7227128
AAF42204.1
7227128
Trx-His
A-06
[157]
936 (GNA2091)
40-78
AAF42408.1 GI:7227353
AAF42408.1
7227353
Trx-His
A-07
[68]
936 (GNA2091)
70-115
AAF42408.1 GI:7227354
AAF42408.1
7227354
Trx-His
A-08
[150]
936 (GNA2091)
125-115; 1-96
AAF42408.1 GI:7227355; AAF42204.1GI: 7227128
AAF42408.1
7227355
Trx-His
A-09
[50]
936 (GNA2091)
173-115; 1-224
AAF42408.1 GI:7227355; AAF42204.1GI: 7227129
AAF42408.1
7227356
Trx-His
A-10
[51]
936 (GNA2091)
179-115; 1-174
AAF42408.1 GI:7227355; AAF42204.1GI: 7227130
AAF42408.1
7227357
His
A-11
[103]
fHbp var1
full length
AAF42204.1GI: 7227128
AAF42204.1
7227128
His
A-12
[153]
fHbp var1
full length
AAF42204.1GI: 7227128
AAF42204.1
7227128
Trx-His
A-13
[155]
fHbp var1
12-100
AAF42204.1GI: 7227128
AAF42204.1
7227128
Trx-His
A-14
[54]
fHbp var1
19-226
AAF42204.1GI: 7227128
AAF42204.1
7227128
His
A-15
[53]
fHbp var1
19-97
AAF42204.1GI: 7227128
AAF42204.1
7227128
His
A-16
[52]
fHbp var1
19-63
AAF42204.1GI: 7227128
AAF42204.1
7227128
His
A-17
[56]
fHbp var1
72-165
AAF42204.1GI: 7227128
AAF42204.1
7227128
His
A-18
[57]
fHbp var1
74-226
AAF42204.1GI: 7227128
AAF42204.1
7227128
His
A-19
[59]
fHbp var1
112-228
AAF42204.1GI: 7227128
AAF42204.1
7227128
His
A-20
106 7 Kbytes .Supplementary data files not provided
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