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Status
Public on Oct 13, 2005
Title
UCSF_Gross_Ecoli_v12.txt
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Escherichia coli Manufacturer
Gross Lab UCSF
Manufacture protocol
PCR products for 4110 ORFs representing 95.8% of the Escherichia coli K12 MG1655 ORFs were prepared according to Richmond et al (1999; Nucleic Acids Res. 27:3821-35). The products were spotted onto glass slides to make DNA arrays as described in MGUIDE at http://cmgm.stanford.edu/pbrown/mguide/index.html and protocols on http://www.microarrays.org/
Support
glass
Coating
polysine
Description
A microarray with 5376 spot features.
Contributor(s)
Rhodius VA Submission date
Oct 10, 2005
Last update date
Oct 13, 2005
Contact name
Virgil Arthur Rhodius
E-mail(s)
[email protected] Phone
1 415 476 1493
URL
http://www.ucsf.edu/gross Organization name
UCSF
Department
Microbiology and Immunology
Lab
Gross
Street address
Genentech Hall, 600 16th Street
City
San Francisco
State/province
CA
ZIP/Postal code
94158-2517
Country
USA
Samples (11)
GSM77348 , GSM77355 , GSM77356 , GSM77357 , GSM77358 , GSM77359
Series (2)
GSE3437 Time course analysis of genes regulated by E. coli sigma E (rpoE) after overexpression
GSE6444 Steady-state analysis of genes regulated by the E. coli RNA chaperone, Hfq
Data table header descriptions ID Block Array spot block coordinate
Row Array spot row coordinate
Column Array spot column coordinate
GODLIST AMAD database unique ID
384 Plate PCR plate identifier
Plate Row PCR 384 well plate row
Plate Column PCR 384 well plate column
clone id Unique ID for gene represented by spot from NCBI (2001) for Escherichia coli K-12 MG1655
ORF Open Reading Frame Identifier
ORF SPOT_ID Name Gene name represented by spot from NCBI (2001) for Escherichia coli K-12 MG1655
Description Description of gene represented by spot from NCBI (2001) for Escherichia coli K-12 MG1655
FAILED PCR fail (1) or success (0)
Data table ID Block Row Column GODLIST 384 Plate Plate Row Plate Column clone id ORF ORF SPOT_ID Name Description FAILED 1_1_1
1
1
1
1
1
A
1
CONTROL
coli genomic 1X
coli genomic 1X
0
1_1_2
1
1
2
5
1
A
5
CONTROL
3X SSC
3X SSC
0
1_1_3
1
1
3
9
1
A
9
CONTROL
coli genomic 1X
coli genomic 1X
0
1_1_4
1
1
4
13
1
A
13
CONTROL
coli genomic 0.5X
coli genomic 0.5X
0
1_1_5
1
1
5
17
1
A
17
CONTROL
3X SSC
3X SSC
0
1_1_6
1
1
6
21
1
A
21
CONTROL
coli genomic 1X
coli genomic 1X
0
1_1_7
1
1
7
97
1
E
1
CONTROL
rDNA 1X
rDNA 1X
0
1_1_8
1
1
8
101
1
E
5
CONTROL
3X SSC
3X SSC
0
1_1_9
1
1
9
105
1
E
9
CONTROL
rDNA 1X
rDNA 1X
0
1_1_10
1
1
10
109
1
E
13
CONTROL
rDNA 0.5X
rDNA 0.5X
0
1_1_11
1
1
11
113
1
E
17
CONTROL
3X SSC
3X SSC
0
1_1_12
1
1
12
117
1
E
21
CONTROL
rDNA 1X
rDNA 1X
0
1_1_13
1
1
13
193
1
I
1
EMPTY
EMPTY
EMPTY
EMPTY
0
1_1_14
1
1
14
197
1
I
5
EMPTY
EMPTY
EMPTY
EMPTY
0
1_1_15
1
1
15
201
1
I
9
EMPTY
EMPTY
EMPTY
EMPTY
0
1_1_16
1
1
16
205
1
I
13
EMPTY
EMPTY
EMPTY
EMPTY
0
1_1_17
1
1
17
209
1
I
17
EMPTY
EMPTY
EMPTY
EMPTY
0
1_1_18
1
1
18
213
1
I
21
EMPTY
EMPTY
EMPTY
EMPTY
0
1_1_19
1
1
19
289
1
M
1
EMPTY
EMPTY
EMPTY
EMPTY
0
1_1_20
1
1
20
293
1
M
5
EMPTY
EMPTY
EMPTY
EMPTY
0
5376 436 Kbytes .Supplementary data files not provided
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