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| Status |
Public on Apr 14, 2023 |
| Title |
Neisseria meningitidis Protein Microarray (Recombinant) |
| Technology type |
spotted peptide or protein |
| Distribution |
non-commercial |
| Organism |
Neisseria meningitidis |
| Manufacturer |
Molecular Biology and Bacteriology lab, GSK, Siena |
| Manufacture protocol |
Purified recombinant protein were diluted at final concentration of 0.5 mg/ml in 40% glycerol and transferred into 384-well plates. Two droplets of 0.1 nL protein solution was deposited per spot on nitrocellulose-coated slides (FAST slides, Maine Manufacturing) using the non-contact printer Marathon Argus Spotter (Arrayjet). Printing was performed in a cabinet with controlled humidity and temperature (50-55% and 18°C, respectively). Each protein was printed in replicates on each slide. Printed protein microarrays were kept at room temperature for 5 hours, dried under a stream of nitrogen and store at 4°C.
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| Description |
spotted microarray containing purified recombinant proteins
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| Submission date |
Feb 02, 2023 |
| Last update date |
Apr 16, 2023 |
| Contact name |
Erika Bartolini |
| Organization name |
GSK Vaccines
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| Department |
AIMB
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| Lab |
Molecular Biology
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| Street address |
Via Fiorentina 1
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| City |
Siena |
| State/province |
Italy |
| ZIP/Postal code |
53100 |
| Country |
Italy |
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| Samples (6) |
GSM7020088, GSM7020089, GSM7020090, GSM7020091, GSM7020092, GSM7020093 |
| Series (2) |
| GSE224340 |
OpcA and PorB are novel bactericidal antigens of the 4CMenB vaccine in mice and humans |
| GSE224348 |
OpcA and PorB are novel bactericidal antigens of the 4CMenB vaccine in mice and humans |
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| Supplementary file |
Size |
Download |
File type/resource |
| GPL33085_Protein_Microarray_annotations.txt.gz |
661 b |
(ftp)(http) |
TXT |
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