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Platform GPL4366 Query DataSets for GPL4366
Status Public on Jan 29, 2008
Title Sunflower organ-specific medium-scale cDNA array
Technology type spotted DNA/cDNA
Distribution custom-commercial
Organism Helianthus annuus
Manufacturer Gentron
Manufacture protocol Five ul from each purified PCR cDNA products (317 in total) were transferred into 384 well plate containing 5 ul of DMSO 100%,spotted onto coated glass-slides in the working solution plates and printed onto UltraGap Slides (Corning) using a Versarray chipwriter (Bio-Rad) with Stealth Micro Spotting quill pins (Telechem). Humidity was set at 45%, and temperature was 22-25°C. Pins were loaded in the working plate during 0.5 sec. Distance between spots was 400 um, Dwell time for printing was 0.05 sec. Four replicas from each cDNA were spotted per slide. Pins were loaded in the working plate and before printing the slides, a blotting slide was printed 5 times in order to discard the excess of cDNA on the pins. After printing on slides, and before loading a new cDNA, pins were washed with 3 cycles of 5 sec. wash with water, followed by 1 sec vacuum and a final 10 sec vacuum time . After printing was completed, cDNA were UV crosslinked to the slides on a CL-1000 UV crosslinker (UVP). Printed slides were stored in a dissecator.
Support glass
Coating aminosilane
 
 
Contributor(s) Fernandez P, Di Rienzo J, Hopp HE, Paniego N, Heinz RA
Submission date Sep 25, 2006
Last update date Jan 29, 2008
Contact name Paula Fernandez
E-mail(s) [email protected]
Organization name INTA Castelar
Street address N. Repetto y Los Reseros S/N
City Castelar
State/province Buenos Aires
ZIP/Postal code 1712
Country Argentina
 
Samples (18) GSM138451, GSM138453, GSM138481, GSM138512, GSM138514, GSM138516 
Series (1)
GSE6201 profiling of an organ-specific sunflower transcriptoma under abiotic stress (salinity and cold conditions)

Data table header descriptions
ID
meta_row Grid row
meta_col Grid column
row1 Spot row within the print grid
col Spot column within array
GB_ACC GenBank accession number of EST used as template to cDNA amplification
Protein_acc
ref EST organ-specific clone name
Tissue Tissue from which each cDNA collection was constructed
BLAST FUNCTION EST putative function based on BLAST algorithm top-hit
SPOT_ID spot identifier

Data table
ID meta_row meta_col row1 col GB_ACC Protein_acc ref Tissue BLAST FUNCTION SPOT_ID
1 1 1 1 1 AAF82805 sunflower sunflower sunflower actin null positive control positive control
2 1 1 1 2 BU671847 T375 T Hypothetical proteins
3 1 1 1 3 BU671881 T450 T Photosynthesis
4 1 1 1 4 16 S Sclerotinia cDNA clone Sclerotinia cDNA clone: 16
5 1 1 1 5 94 S Sclerotinia cDNA clone Sclerotinia cDNA clone: 94
6 1 1 1 6 BU671840 T318 T Defense / Stress related proteins
7 1 1 1 7 EMPTY null null -- EMPTY
8 1 1 1 8 EMPTY null null -- EMPTY
9 1 1 2 1 BU672064 H202 H Photosynthesis
10 1 1 2 2 BU672081 H312 H Photosynthesis
11 1 1 2 3 BU672090 H385 H Secondary metabolism
12 1 1 2 4 BU672097 H448 H EST sequences
13 1 1 2 5 BU671803 T113 T Defense / Stress related proteins
14 1 1 2 6 BU671813 T166 T Ribosomal protein
15 1 1 2 7 BU671826 T222 T Hypothetical proteins
16 1 1 2 8 BU671871 T269 T Hypothetical proteins
17 1 1 3 1 BU671842 T338 T Ribosomal
18 1 1 3 2 BU671850 T387 T Transport
19 1 1 3 3 BU671860 T464 T DNA synthesis
20 1 1 3 4 33 S Sclerotinia cDNA clone Sclerotinia cDNA clone: 33

Total number of rows: 1536

Table truncated, full table size 74 Kbytes.




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