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Platform GPL4604 Query DataSets for GPL4604
Status Public on Dec 01, 2006
Title CUFSL Listeria interalins 384 v1
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Listeria monocytogenes
Manufacturer Big Red Spots, T2 002B Vet Research Tower, Cornell university, Ithaca NY 14853 USA
Manufacture protocol Oligonucleotides were resuspended in 1× SSC, 0.0025% Sarcosyl to a final molarity of 30uM. All oligonucleotides and controls were spotted in duplicate on Amino-silane coated Corning UltraGAPS slides (Corning, NY USA) using a custom built XYZ microarrayer. Oligonucleotides were UV-crosslinked at 300mJ for 1 minute. Printing quality was checked using Cy3-labeled random sequence nonamers
Support glass
Coating aminosilane
 
 
Contributor(s) McGann P, Wiedmann M, Boor KJ
Submission date Nov 29, 2006
Last update date Dec 01, 2006
Contact name Patrick Mc Gann
E-mail(s) [email protected]
Phone (607) 227 0627
Organization name Cornell University
Department Food Science
Lab Wiedmann
Street address 412 Stocking Hall
City Ithaca
State/province NY
ZIP/Postal code 14850
Country USA
 
Samples (7) GSM148005, GSM148025, GSM148033, GSM148040, GSM148833, GSM148839 
Series (2)
GSE6421 regulation of Listeria monocytogenes internalin-like genes by σB and PrfA as revealed by subgenomic microarray analyses
GSE6471 Temperature-dependent expression of internalin and internalin-like genes

Data table header descriptions
ID
Block Block number
Row Row number
Column Column number
Type hk=Housekeeper; test=Test gene; PC=positive control; bc=Background control
ORF
SPOT_ID
SEQUENCE Sequence of Oligo based on L. monocytogenes EDGe genome (Accession no.:AL591824)

Data table
ID Block Row Column Type ORF SPOT_ID SEQUENCE
accA95a 1 1 1 hk AAGAAATTAACAAAACTATCACGAAGTCTTTACATGCACTAATGGCATTTTCTGAAGAACAATTAATAAA
accA95b 1 1 2 hk AAGAAATTAACAAAACTATCACGAAGTCTTTACATGCACTAATGGCATTTTCTGAAGAACAATTAATAAA
accA46a 1 1 3 hk TTCTGAAGAACAATTAATAAAACAACGTTATGAAAAATTCAAGAAAATCGGTGTATATGAGACTATATAA
accA46b 1 1 4 hk TTCTGAAGAACAATTAATAAAACAACGTTATGAAAAATTCAAGAAAATCGGTGTATATGAGACTATATAA
alaS1628a 1 1 5 hk GCAAAAGTTCTAACAATCAACGAACCATTTATGTACAAATTAGTACCAGTAGTTGGGAAAATCATGAATA
alaS1628b 1 1 6 hk GCAAAAGTTCTAACAATCAACGAACCATTTATGTACAAATTAGTACCAGTAGTTGGGAAAATCATGAATA
alaS2573a 1 1 7 hk AAAATGAAACAATTATCAAGCGCTGAAGTAAGACAGTTATTTTTAGATTTCTTTAAAGAAAAAGGGCATA
alaS2573b 1 1 8 hk AAAATGAAACAATTATCAAGCGCTGAAGTAAGACAGTTATTTTTAGATTTCTTTAAAGAAAAAGGGCATA
appC405a 1 1 9 hk ATATAGTGATGCGTTTTCTTGACATTTTCATGTCGATTCCGTCATTTTTCCTACTAATGATTTTAAATGC
appC405b 1 1 10 hk ATATAGTGATGCGTTTTCTTGACATTTTCATGTCGATTCCGTCATTTTTCCTACTAATGATTTTAAATGC
appC463a 1 1 11 hk GATTTTAAATGCATACTTAAAACCGGGAATTTCGAACATTATTATCATTATCGGATTGCTTTCTTGGATG
appC463b 1 1 12 hk GATTTTAAATGCATACTTAAAACCGGGAATTTCGAACATTATTATCATTATCGGATTGCTTTCTTGGATG
aroE498a 1 2 1 hk TACGCGAATGCAGAAAAAACAGTAGAAAAAATCAATTCGAAAACAGATTGTAAAGCACAATTATTTGATA
aroE498b 1 2 2 hk TACGCGAATGCAGAAAAAACAGTAGAAAAAATCAATTCGAAAACAGATTGTAAAGCACAATTATTTGATA
aroE241a 1 2 3 hk CATAAATACTTAGATAAACTTTCTCCAGCCGCTGAACTTGTAGGGGCAGTAAATACAGTTGTTAATGATG
aroE241b 1 2 4 hk CATAAATACTTAGATAAACTTTCTCCAGCCGCTGAACTTGTAGGGGCAGTAAATACAGTTGTTAATGATG
aspB580a 1 2 5 hk AATATGATTTCTTTATTTTATCGGATGAAGTATATGATGGTTTTAGTTTTTATGAAGACTTCGTACCAAT
aspB580b 1 2 6 hk AATATGATTTCTTTATTTTATCGGATGAAGTATATGATGGTTTTAGTTTTTATGAAGACTTCGTACCAAT
aspB452a 1 2 7 hk TAATGTTGATATTTTAGAAGCAGCCATTACTGATAAAACAAAAGCTTTAATTTTAAATTCACCTAATAAT
aspB452b 1 2 8 hk TAATGTTGATATTTTAGAAGCAGCCATTACTGATAAAACAAAAGCTTTAATTTTAAATTCACCTAATAAT

Total number of rows: 384

Table truncated, full table size 26 Kbytes.




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