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Platform GPL4620 Query DataSets for GPL4620
Status Public on Jan 01, 2007
Title UCSF_Gross_Ecoli_v19.txt
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Escherichia coli
Manufacturer Gross Lab UCSF
Manufacture protocol PCR products for 4110 ORFs representing 95.8% of the Escherichia coli K12 MG1655 ORFs were prepared according to Richmond et al (1999; Nucleic Acids Res. 27:3821-35). The products were spotted onto glass slides to make DNA arrays as described in MGUIDE at http://cmgm.stanford.edu/pbrown/mguide/index.html and protocols on http://www.microarrays.org/
Support glass
Coating polysine
 
Description A microarray with 5760 spot features.
Tip configuration: Standard 16-tip steel pin
Columns per Sector: 20
Rows per Sector: 18
Column spacing: 210 um
Row spacing: 210 um
 
Contributor(s) Guisbert E, Rhodius VA, Ahuja N, Witkin E, Gross CA
Submission date Dec 01, 2006
Last update date Jan 01, 2007
Contact name Virgil Arthur Rhodius
E-mail(s) [email protected]
Phone 1 415 476 1493
URL http://www.ucsf.edu/gross
Organization name UCSF
Department Microbiology and Immunology
Lab Gross
Street address Genentech Hall, 600 16th Street
City San Francisco
State/province CA
ZIP/Postal code 94158-2517
Country USA
 
Samples (6) GSM147988, GSM147989, GSM147994, GSM147995, GSM147996, GSM147997
Series (1)
GSE6444 Steady-state analysis of genes regulated by the E. coli RNA chaperone, Hfq

Data table header descriptions
ID
Block Array spot block coordinate
Row Array spot row coordinate
Column Array spot column coordinate
384 Plate PCR plate identifier
Plate Row PCR 384 well plate row
Plate Column PCR 384 well plate column
NOMAD_ID Unique ID on UCSF NOMAD database
ORF Clone ID; unique ID for gene represented by spot from NCBI (2004) for Escherichia coli K-12 MG1655
ORF_TYPE Open Reading Frame Identifier
Name Gene name represented by spot from NCBI (2004) for Escherichia coli K-12 MG1655
Description Description of gene represented by spot from NCBI (2004) for Escherichia coli K-12 MG1655
FAILED PCR fail (1) or success (0)
SPOT_ID spot identifier

Data table
ID Block Row Column 384 Plate Plate Row Plate Column NOMAD_ID ORF ORF_TYPE Name Description FAILED SPOT_ID
1_1_1 1 1 1 1 D 1 73_b0570 b0570 ORF ybcZ putative 2-component sensor protein 0
1_1_2 1 1 2 1 D 5 77_b1284 b1284 ORF b1284 putative DEOR-type transcriptional regulator 0
1_1_3 1 1 3 1 D 9 81_b1827 b1827 ORF b1827 putative regulator 0
1_1_4 1 1 4 1 D 13 85_b2380 b2380 ORF b2380 putative sensor protein 0
1_1_5 1 1 5 1 D 17 89_b2852 b2852 ORF ygeH putative invasion protein 0
1_1_6 1 1 6 1 D 21 93_FAIL ORF yhhM putative receptor 1 FAIL_yhhM
1_1_7 1 1 7 1 H 1 169_b0603 b0603 ORF ybdO putative transcriptional regulator LYSR-type 0
1_1_8 1 1 8 1 H 5 173_b1328 b1328 ORF ycjZ putative transcriptional regulator LYSR-type 0
1_1_9 1 1 9 1 H 9 177_FAIL ORF cbl transcriptional regulator cys regulon; accessory regulatory circuit affecting cysM 1 FAIL_cbl
1_1_10 1 1 10 1 H 13 181_FAIL ORF yfeR putative transcriptional regulator LYSR-type 1 FAIL_yfeR
1_1_11 1 1 11 1 H 17 185_b2921 b2921 ORF ygfI putative transcriptional regulator LYSR-type 0
1_1_12 1 1 12 1 H 21 189_b3487 b3487 ORF yhiI putative membrane protein 0
1_1_13 1 1 13 1 L 1 265_b0620 b0620 ORF citB sequence similarity to Shigella regulator 0
1_1_14 1 1 14 1 L 5 269_b1384 b1384 ORF feaR regulatory protein for 2-phenylethylamine catabolism 0
1_1_15 1 1 15 1 L 9 273_b2015 b2015 ORF yeeY putative transcriptional regulator LYSR-type 0
1_1_16 1 1 16 1 L 13 277_FAIL ORF yfeU putative regulator 1 FAIL_yfeU
1_1_17 1 1 17 1 L 17 281_b3025 b3025 ORF ygiX putative 2-component transcriptional regulator 0
1_1_18 1 1 18 1 L 21 285_FAIL ORF yhiX putative ARAC-type regulatory protein 1 FAIL_yhiX
1_1_19 1 1 19 1 P 1 361_b0768 b0768 ORF ybhD putative transcriptional regulator LYSR-type 0
1_1_20 1 1 20 1 P 5 365_b1439 b1439 ORF b1439 multi modular; putative transcriptional regulator; also putative ATP-binding component of a transport system 0

Total number of rows: 5760

Table truncated, full table size 480 Kbytes.




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