Edinburgh Research and Innovation, The University of Edinburgh
Manufacture protocol
Researchers at the University of Edinburgh have constructed a microarray to allow investigation into a large set of immuno-inflammatory genes regulated in response to disease, vaccination and therapeutic interventions in ruminants. Specifically designed to investigate the host response to infection by Mycobacterium avium subsp. paratuberculosis (Johne's disease), the microarray can also be applied to other disease states in ruminants. The array will also provide information on selectable traits that may confer resistance to Johne's disease and responses to vaccination. The gene array consists of approximately 600 ruminant immuno-modulatory genes backed up by a database and management system to allow full interrogation of experimental data. The full array, or RIGUA (Ruminant Immuno-inflammatory Gene Universal Array), consists of some 600 ruminant genes each represented by two non-overlapping 75-mer oligonucleotides. These have been selected to enable investigations into the immune response to infection and other challenges, including vaccination, in ruminants. It will therefore be useful in defining those genes involved in the development of disease, which have an immuno-inflammatory component thus contributing to animal improvement and breeding. It will also be useful in vaccine and adjuvant response studies in the development of vaccines to ruminant disease.
Description
For additional information please contact Prof. John Hopkins or S. Rhind at The University of Edinburgh
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