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| Status |
Public on Aug 17, 2017 |
| Title |
Next-generation sequencing measuring m6A enrichment in CD4+ T cells isolated from WT mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CD4+ T cells were isolated by StemCell CD4+ T cell isolation kit of spleen and lymph nodes WT mice. Total RNAs were isolated from the pure CD4+ T cells, subjected to standard m6A RIP protocol. The libraries were generated with IPed and Input RNAs and subjected to sequencing. Raw sequencing reads were aligned to the mouse genome (mm10) with Tophat, and gene expression levels were measured by Cufflinks.
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| Overall design |
m6A modified RNAs were immunoprecipitated and enriched with m6A specific antibody from WT mouse CD4+ T cells, and subjected to deep sequencing, in duplicate, using Illumina HiSeq 2000.
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| Contributor(s) |
Flavell RA, Li H |
| Citation(s) |
28792938 |
| |
| Submission date |
Jun 21, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Huabing Li |
| E-mail(s) |
[email protected]
|
| Phone |
7326668951
|
| Organization name |
Yale University School of Medicine
|
| Department |
Immunobiology
|
| Street address |
1 Gilbert Street, S570
|
| City |
New Haven |
| State/province |
CONNECTICUT |
| ZIP/Postal code |
06519 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE100048 |
m6A mRNA methylation controls T cell homeostasis by targeting IL-7/STAT5/SOCS pathway |
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| Relations |
| BioProject |
PRJNA391349 |
| SRA |
SRP110046 |
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