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| Status |
Public on Jun 28, 2017 |
| Title |
Whole genome ChIP-seq of histone H3 threonine 11 phosphorylation in Saccharomyces cerevisiae |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We used ChIP-seq to determine the whole-genome enrichment of histone H3 threonine 11 phosphorylation (H3 T11ph) during Saccharomyces cerevisiae meiosis. S. cerevisiae SK1 cells were synchronized for meiotic entry and 3 and 4 hour meiotic samples were obtained. As H3 T11ph is dependent on the formation of meiotic double strand breaks (DSBs), a negative control ChIP-seq sample was obtained from a strain lacking DSBs (spo11-yf). Concurrently, ChIP-seq was carried out for histone H3 as a control for comparision.
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| Overall design |
Ten ChIP-seq samples total were taken from the following conditions: Anti-H3 T11ph and anti-H3, 3 and 4 hours synchronous meiosis, biological replicates from wild type strain (8 samples). Anti-H3 T11ph and anti-H3, 3.5 hours synchronous meiosis single sample from spo11-Y135F DSB-negative mutant (2 samples).
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| Contributor(s) |
Murakami H, Kniewel R, Keeney S |
| Citation(s) |
28986445 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM058673 |
Mechanism of meiotic recombination in yeast |
Sloan Kettering Institute for Cancer Research |
Scott Keeney |
| R35 GM118092 |
Mechanism and regulation of meiotic recombination |
Sloan Kettering Institute for Cancer Research |
Scott Keeney |
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| Submission date |
Jun 27, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Ryan Kniewel |
| Organization name |
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CIB-CSIC)
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| Department |
Department of Environmental Biology
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| Street address |
calle Ramiro de Maeztu, 9
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA392121 |
| SRA |
SRP110610 |
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