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| Status |
Public on Jan 17, 2019 |
| Title |
Single Cell RNA-seq for porcine oocytes |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the application of single cell transcriptome sequencing technology for high-throughput profiling of the brilliant cresyl blue test-positive porcine oocytes had higher rates of meiotic maturation, lower death rates, and better cleavage and blastocyst rates as well. Single oocyte transcriptome sequencing on porcine germinal vesicle (GV) stage oocytes that differentially stained by BCB identified 155 genes with significant abundance differences, including CDC5L, LDHA, SPATA22, RGS2, PAIP1, WEE1B and HSP27, which enriched in functionally important signaling pathways, such as spliceosome, cell cycle, oocyte meiosis, and nucleotide excision repair.
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| Overall design |
Assays on three BCB-positive and three BCB-negative GV porcine oocytes.
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| Contributor(s) |
Yang C, Du Z |
| Citation(s) |
29222335 |
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| Submission date |
Jul 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Zhi-Qiang Du |
| E-mail(s) |
[email protected]
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| Phone |
86-451-55191805
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| Organization name |
Northeast Agricultural University
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| Street address |
59 Mucai Street, XiangFang District
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| City |
Harbin |
| ZIP/Postal code |
150030 |
| Country |
China |
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| Platforms (1) |
| GPL9126 |
Illumina Genome Analyzer (Sus scrofa) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA393847 |
| SRA |
SRP111540 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE101181_RAW.tar |
2.8 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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