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| Status |
Public on Jan 23, 2008 |
| Title |
Identification of genes responsive to ultrasound in human leukemia U937 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Here, the effects of non-thermal low intensity pulsed ultrasound (LIPUS) on the gene expression in human lymphoma U937 cells were investigated using by an Affymetrix GeneChip system. Six hours after LIPUS treatment (0.3 W/cm2 for 1 min), apoptosis (14±3.8%, mean±SD) with minimal cell lysis was observed. At 3 h post-treatment, LIPUS down-regulated 193 genes and up-regulated 201 genes by >1.5-fold.
Keywords: ultrasound, gene expression, Human lymphoma U937 cell
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| Overall design |
U937 cells, a human lymphoma cell line, were treated with low intensity pulsed ultrasound (0.3 W/cm2 for 1 min) and followed by incubation for 3 h at 37°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with Human Expression Array U133A which was spotted with 22,283 probe sets. Sample preparation for array hybridization was carried out as described in the manufacture’s instructions.
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| Contributor(s) |
Tabuchi Y, Takasaki I, Kondo T |
| Citation(s) |
18571840 |
| |
| Submission date |
Jan 18, 2008 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Yoshiaki Tabuchi |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Toyama
|
| Department |
Life Science Research Center
|
| Lab |
Division of Molecular Genetics Research
|
| Street address |
2630 Sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (4)
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| GSM257871 |
U937 cells treated with ultrasound (control-1) |
| GSM257872 |
U937 cells treated with ultrasound (control-2) |
| GSM257873 |
U937 cells treated with ultrasound (treatment-1) |
| GSM257874 |
U937 cells treated with ultrasound (treatment-2) |
|
| Relations |
| BioProject |
PRJNA108333 |
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