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| Status |
Public on Nov 30, 2017 |
| Title |
Expression data from WT and AIMp1KO mouse bone marrow-derived dendritic cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dendritic cells are critical regulators of adaptive immune responses which can process and present antigens to effector cells. As a structural component of multi-enzyme aminoacyl tRNA synthetase complex, AIMp1 (p43) is identified to promote type 1 T-cell responses by DCs and play important roles in both antitumor and antiviral immunity.
We use high-throughput microarray analysis to compare the gene expression profiles of WT vs. AIMp1KO bone marrow-derived DCs (BMDC) to study the mechanism by which DC-expressed AIMp1 promote type 1 T-cell immune responses.
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| Overall design |
WT or AIMp1KO BMDCs were loaded with antigens and matured for RNA extraction and hybridization on Affymetrix microarrays. Cells were unloaded or loaded with OVA protein and SIINFEKL peptide (10ug/mL) for 3 hours in media containing 5% serum; then matured with cytokine cocktail (10ng/mL TNFa, 15ng/mL IL-6, 10ng/mL IL-1b, 1ug/mL PGE2) for 48hours prior to harvest.
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| Contributor(s) |
Liang D, Decker WK |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Aug 04, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Dan Liang |
| Organization name |
Baylor college of medicine
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| Department |
Pathology and Immunology
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| Street address |
One baylor plaza, Room N920
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| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030-3498 |
| Country |
USA |
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| Platforms (1) |
| GPL20775 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CSV version] |
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| Samples (12)
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| GSM2732842 |
WT BMDC loaded with OVA+SIINFEKL and matured, biological rep 1 |
| GSM2732843 |
WT BMDC loaded with OVA+SIINFEKL and matured, biological rep 2 |
| GSM2732844 |
WT BMDC loaded with OVA+SIINFEKL and matured, biological rep 3 |
| GSM2732845 |
AIMp1KO BMDC matured only, biological rep 1 |
| GSM2732846 |
AIMp1KO BMDC matured only, biological rep 2 |
| GSM2732847 |
AIMp1KO BMDC matured only, biological rep 3 |
| GSM2732848 |
AIMp1KO BMDC loaded with OVA+SIINFEKL and matured, biological rep 1 |
| GSM2732849 |
AIMp1KO BMDC loaded with OVA+SIINFEKL and matured, biological rep 2 |
| GSM2732850 |
AIMp1KO BMDC loaded with OVA+SIINFEKL and matured, biological rep 3 |
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| Relations |
| BioProject |
PRJNA397213 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE102282_RAW.tar |
387.4 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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