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| Status |
Public on Oct 06, 2017 |
| Title |
RNA sequencing analysis of triple cytokine-captured human CD4 T cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
GM-CSF positve CD4 cells are found at sites of inflammation. The purpose of this study was to understand their transcriptional profile relative to known Th1 and Th17 subsets.
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| |
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| Overall design |
Human CD4 T cells were isolated by magnetic negative selection and activated with PMA and ionomycin. A cytokine capture assay was used to isolate CD45RA-positive, cytokine negative, IFN-gamma-single-positive, IL-17A-single-positive, GM-CSF-single positive and IL-17A-GM-CSF-double positive cells.
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| |
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| Contributor(s) |
Al-Mossawi MH, Fang H, Bowness P |
| Citation(s) |
29142230 |
| |
| Submission date |
Sep 16, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Mohammad Hussein Al-Mossawi |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Oxford
|
| Department |
Nuffield Department of Orthopaedics Rheumatology and Musculoskeletal sciences
|
| Lab |
Bowness
|
| Street address |
Botnar Research Centre
|
| City |
Oxford |
| ZIP/Postal code |
OX3 7LD |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA407635 |
| SRA |
SRP117785 |
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