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| Status |
Public on Sep 27, 2017 |
| Title |
Expression data from retinoic acid-induced differentiation of human embryonic stem cells (hESCs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human embryonic stem cells (hESCs) display a unique ability to self-renew in culture while retaining the potential to form the full repertoire of cell types found in the body. A complex molecular network underlies the maintenance of this pluripotent state.
We used microarrays to identify genes that are highly expressed in pluripotent hESCs, but are consequently down-regulated upon differentiation in the presence of retinoic acid.
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| Overall design |
H1 hESCs in mTeSR media were differentiated in 5 μM retinoic acid. RNA samples were collected at daily timepoints (days 0 to 10 of differentiation) for hybridization on Affymetrix microarrays. Undifferentiated HUES8 and H9 hESC lines were maintained in mTeSR media and anaylzed for control and comparison purposes.
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| Contributor(s) |
Ivanova N, Wang Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 23, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Natalia Ivanova |
| Organization name |
Yale University
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| Department |
Genetics
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| Lab |
Ivanova Lab
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| Street address |
10 Amistad Street, Room 220
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06519 |
| Country |
USA |
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| Platforms (2) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL97 |
[HG-U133B] Affymetrix Human Genome U133B Array |
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| Samples (52)
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| GSM2791234 |
HUES8, chip HG-U133B, biological rep2 |
| GSM2791235 |
H9, chip HG-U133A, biological rep1 |
| GSM2791236 |
H9, chip HG-U133B, biological rep1 |
| GSM2791237 |
H9, chip HG-U133A, biological rep2 |
| GSM2791238 |
H9, chip HG-U133B, biological rep2 |
| GSM2791239 |
H1 at D0, chip HG-U133A, biological rep1 |
| GSM2791240 |
H1 at D0, chip HG-U133B, biological rep1 |
| GSM2791241 |
H1 at D0, chip HG-U133A, biological rep2 |
| GSM2791242 |
H1 at D0, chip HG-U133B, biological rep2 |
| GSM2791243 |
H1 at D1, chip HG-U133A, biological rep1 |
| GSM2791244 |
H1 at D1, chip HG-U133B, biological rep1 |
| GSM2791245 |
H1 at D1, chip HG-U133A, biological rep2 |
| GSM2791246 |
H1 at D1, chip HG-U133B, biological rep2 |
| GSM2791247 |
H1 at D2, chip HG-U133A, biological rep1 |
| GSM2791248 |
H1 at D2, chip HG-U133B, biological rep1 |
| GSM2791249 |
H1 at D2, chip HG-U133A, biological rep2 |
| GSM2791250 |
H1 at D2, chip HG-U133B, biological rep2 |
| GSM2791251 |
H1 at D3, chip HG-U133A, biological rep1 |
| GSM2791252 |
H1 at D3, chip HG-U133B, biological rep1 |
| GSM2791253 |
H1 at D3, chip HG-U133A, biological rep2 |
| GSM2791254 |
H1 at D3, chip HG-U133B, biological rep2 |
| GSM2791255 |
H1 at D4, chip HG-U133A, biological rep1 |
| GSM2791256 |
H1 at D4, chip HG-U133B, biological rep1 |
| GSM2791257 |
H1 at D4, chip HG-U133A, biological rep2 |
| GSM2791258 |
H1 at D4, chip HG-U133B, biological rep2 |
| GSM2791259 |
H1 at D5, chip HG-U133A, biological rep1 |
| GSM2791260 |
H1 at D5, chip HG-U133B, biological rep1 |
| GSM2791261 |
H1 at D5, chip HG-U133A, biological rep2 |
| GSM2791262 |
H1 at D5, chip HG-U133B, biological rep2 |
| GSM2791263 |
H1 at D6, chip HG-U133A, biological rep1 |
| GSM2791264 |
H1 at D6, chip HG-U133B, biological rep1 |
| GSM2791265 |
H1 at D6, chip HG-U133A, biological rep2 |
| GSM2791266 |
H1 at D6, chip HG-U133B, biological rep2 |
| GSM2791267 |
H1 at D7, chip HG-U133A, biological rep1 |
| GSM2791268 |
H1 at D7, chip HG-U133B, biological rep1 |
| GSM2791269 |
H1 at D7, chip HG-U133A, biological rep2 |
| GSM2791270 |
H1 at D7, chip HG-U133B, biological rep2 |
| GSM2791271 |
H1 at D8, chip HG-U133A, biological rep1 |
| GSM2791272 |
H1 at D8, chip HG-U133B, biological rep1 |
| GSM2791273 |
H1 at D8, chip HG-U133A, biological rep2 |
| GSM2791274 |
H1 at D8, chip HG-U133B, biological rep2 |
| GSM2791275 |
H1 at D9, chip HG-U133A, biological rep1 |
| GSM2791276 |
H1 at D9, chip HG-U133B, biological rep1 |
| GSM2791277 |
H1 at D9, chip HG-U133A, biological rep2 |
| GSM2791278 |
H1 at D9, chip HG-U133B, biological rep2 |
| GSM2791279 |
H1 at D10, chip HG-U133A, biological rep1 |
| GSM2791280 |
H1 at D10, chip HG-U133B, biological rep1 |
| GSM2791281 |
H1 at D10, chip HG-U133A, biological rep2 |
| GSM2791282 |
H1 at D10, chip HG-U133B, biological rep2 |
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| Relations |
| BioProject |
PRJNA412209 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE104173_RAW.tar |
109.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| GSE104173_RAdiff_Matrix.txt.gz |
7.2 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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