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| Status |
Public on Feb 01, 2018 |
| Title |
Lung Epithelial Response to Cigarette Smoke and Modulation by the Nicotinic Alpha 7 Receptor |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the impact of side-stream cigarette smoking on baseline tracriptional status of enriched epithelium from the distal lung of both male and female control mice or mice harboring a mutation in the nicotinc alpha7 recptor that selectivley diminshes the calcium current (E260A).
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| Overall design |
Mice (male or female) of each nicotinic recptor alpha7 genotype (Control (c) or mutant (E260A)) were exposed to side-stream cigarette smoke 5 days per week for four months. The distal lung epithelium was enriched and poly-adenylated strand-specific RNA-Seq libraries using Illumina TruSeq stranded mRNA were preared for analysis.
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| Contributor(s) |
Rogers S |
| Citation(s) |
29117258 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL135610 |
Aerosolized Nicotine Modulation of Host Inflammation and Microbiota Dysbiosis |
UNIVERSITY OF UTAH |
SCOTT Warren ROGERS |
| P01 CA098262 |
Social - Emotional Contexts of Adolescent Smoking Patterns |
BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS |
ROBIN J. MERMELSTEIN |
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| |
| Submission date |
Oct 17, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Robert B Weiss |
| E-mail(s) |
[email protected]
|
| Phone |
(801) 585-5606
|
| Organization name |
University of Utah
|
| Department |
Human Genetics
|
| Lab |
EIHG, Rm 2260B
|
| Street address |
15 N 2030 E
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA414619 |
| SRA |
SRP120168 |
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