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| Status |
Public on May 31, 2008 |
| Title |
Mfaveolata time course bleaching experiment |
| Organism |
Orbicella faveolata |
| Experiment type |
Expression profiling by array
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| Summary |
The declining health of coral reefs worldwide is likely to intensify in response to continued anthropogenic disturbance from coastal development, pollution, and climate change. In response to these stresses, reef-building corals may exhibit bleaching, which marks the breakdown in symbiosis between coral and zooxanthellae. Mass coral bleaching due to elevated water temperature can devastate coral reefs on a large geographic scale. In order to understand the molecular and cellular basis of bleaching in corals, we have measured gene expression changes associated with thermal stress and bleaching using a cDNA microarray containing 1,310 genes of the Caribbean coral Montastraea faveolata. In a first experiment, we identified differentially expressed genes by comparing experimentally bleached M. faveolata fragments to control non-heat-stressed fragments. We also identified differentially expressed genes during a time course experiment with four time points across nine days. Results suggest that thermal stress and bleaching in M. faveolata affect the following processes: oxidative stress, Ca2+ homeostasis, cytoskeletal organization, cell death, calcification, metabolism, protein synthesis, heat shock protein activity, and transposon activity. These results represent the first large-scale transcriptomic study focused on revealing the cellular foundation of thermal stress-induced coral bleaching. We postulate that oxidative stress in thermal-stressed corals causes a disruption of Ca2+ homeostasis, which in turn leads to cytoskeletal and cell adhesion changes, decreased calcification, and the initiation of cell death via apoptosis and necrosis.
Keywords: thermal stress response, time course, coral bleaching
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| Overall design |
Time course with 4 time points and 4 biological replicates per time point. Each biological replicate at each time point was hybridized to a pooled reference control sample containing RNA from all control non-heat-stressed coral fragments.
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| Contributor(s) |
Voolstra CR, Sunagawa S, Schwarz JA, Stillman JH, Co MA, Szmant AM, Medina M |
| Citation(s) |
18662230 |
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| Submission date |
Feb 25, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Michael DeSalvo |
| E-mail(s) |
[email protected]
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| Organization name |
UC San Francisco
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| Department |
Department of Anesthesia
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| Lab |
Roland Bainton
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| Street address |
600 16 St Box 2200
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158-2200 |
| Country |
USA |
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| Platforms (1) |
| GPL6515 |
UCM-Medina-Montastraea faveolata-1310-v1 |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE10680 |
Montastraea faveolata bleaching study |
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| Relations |
| BioProject |
PRJNA108843 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE10632_RAW.tar |
4.6 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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