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| Status |
Public on Mar 24, 2020 |
| Title |
MS2-affinity purification coupled to RNA-sequencing (MAPS) reveals targetomes of Z. mobilis sRNAs Zms4 and Zms6 |
| Organism |
Zymomonas mobilis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The MS2-affinity purification coupled to RNA-sequencing (MAPS) approach was used to identify RNA targets that physically associate in vivo with Zymomonas mobilis small RNAs Zms4 and Zms6.
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| Overall design |
RNA-sequencing of affinity-purified in vivo regulatory complexes in three Z. mobilis strains containing plasmids expressing MS2-Zms4, MS2-Zms6, and MS2 alone. MS2-Zms4 and MS2-Zms6 were each compared to MS2 alone as a control, each with biological triplicates.
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| |
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| Contributor(s) |
Haning K, Cho S, Gonzalez-Rivera JC, Simonsen BA, Contreras LM |
| Citation(s) |
32195236 |
| |
| Submission date |
Nov 20, 2017 |
| Last update date |
Mar 24, 2020 |
| Contact name |
Lydia M Contreras |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Texas at Austin
|
| Department |
McKetta Department of Chemical Engineering
|
| Street address |
200 E. Dean Keeton St Stop C0400
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24294 |
Illumina NextSeq 500 (Zymomonas mobilis) |
|
| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE107219 |
Mapping regulatory networks of Zymomonas mobilis small RNAs Zms4 and Zms6 |
|
| Relations |
| BioProject |
PRJNA419246 |
| SRA |
SRP125351 |
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