|
| Status |
Public on Jul 18, 2018 |
| Title |
Study of ribosome dynamics after osmotic stress in Lsm1 and Pat1 deletions |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
In this study, we aim to understand how the fates of stress-activated mRNAs are determined under stress conditions by isolating individual osmostress-activated mRNA species, quantitating the proteins associated in vivo with each of them, and analyzing how deletion of these proteins impact on the expression of stress-activated genes. By comparison with the proteome associated with individual not stress-related mRNA species, we show specific proteins to be preferentially binding to osmostress-activated mRNAs, notably members of the cytoplasmic Pat1 / Lsm1 – 7 complex. We evaluated the impact of this complex on the stress response by analyzing expression of osmostress-activated genes, production of osmo-protein and, mapping ribosome transit at single nucleotide resolution using 5’-phosphate sequencing of mRNAs in lsm1 and pat1 mutants. We conclude that our biochemical co-purification approach has successfully identified RNA-binding proteins with a particular role in regulating post-transcriptional expression of stress-activated mRNAs.
|
| |
|
| Overall design |
We performed 3 biologically independent 5PSeq experiments before stress (t0) and 30 minutes after osmotic stress (t 30).
|
| |
|
| Contributor(s) |
Garre E, Pelechano V, Sunnerhagen P |
| Citation(s) |
30059503 |
| |
| Submission date |
Nov 21, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Vicent Pelechano |
| E-mail(s) |
[email protected]
|
| Organization name |
ScilifeLab - Karolinska Institutet
|
| Department |
MTC
|
| Street address |
Nobels väg 16
|
| City |
Solna |
| ZIP/Postal code |
SE-17177 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA419414 |
| SRA |
SRP125436 |
Less...
More...