|
| Status |
Public on May 01, 2018 |
| Title |
Co-transcriptional association of Hfq and Crc with the Pseudomonas aeruginosa PAO1 genome |
| Organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Hfq is an RNA chaperone and an important post-transcriptional regulator in bacteria. Using chromatin immunoprecipitation together with DNA sequencing (ChIP-Seq), we show that Hfq associates with hundreds of different regions of the Pseudomonas aeruginosa chromosome. These associations are abolished when transcription is inhibited, indicating they reflect Hfq binding to transcripts during their synthesis. Analogous ChIP-Seq analyses with the post-transcriptional regulator Crc reveal that it associates with many of the same nascent transcripts as Hfq, an activity we show is Hfq dependent. Our findings indicate that Hfq binds many transcripts co-transcriptionally in P. aeruginosa, often in concert with Crc, and uncover direct regulatory targets of these proteins. They also highlight a general approach for studying the interactions of RNA-binding proteins with nascent transcripts in bacteria. The binding of post-transcriptional regulators to nascent mRNAs may represent a prevalent means of controlling translation in bacteria where transcription and translation are coupled.
|
| |
|
| Overall design |
Genome-wide locations of Hfq and Crc with and without rifampicin treatment
|
| |
|
| Contributor(s) |
Kambara TK, Ramsey KM, Dove SL |
| Citation(s) |
29719264 |
| BioProject |
PRJNA428223 |
| |
| Submission date |
Feb 23, 2018 |
| Last update date |
Jul 31, 2018 |
| Contact name |
Simon L Dove |
| E-mail(s) |
[email protected]
|
| Organization name |
Boston Children's Hospital and Harvard Medical School
|
| Department |
Division of Infectious Diseases
|
| Lab |
Dove Laboratory
|
| Street address |
300 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18782 |
Illumina HiSeq 2500 (Pseudomonas aeruginosa PAO1) |
|
| Samples (20)
|
|
| Relations |
| SRA |
SRP127958 |
Less...
More...