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| Status |
Public on Aug 05, 2018 |
| Title |
ATAC sequencing of HAP1 cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We analysed the global effect of CHD3-KO and SENP1-KO HAP1 cell lines compared to control cell line, on chromatin accessibility using ATAC-seq.
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| Overall design |
CRISPR-Cas9-generated knockout cells for CHD3 and SENP1 in HAP1 cells were assayed by ATAC-seq, where 150,000 cells were harvested to investigate changes in chromatin accessibility. Three individual experiments were performed. Libraries were prepared and 40-bp paired-end reads were obtained using an Illumina Nextseq 500 sequencer.
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| |
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| Contributor(s) |
Gabrielsen OS, Lemma RB |
| Citation(s) |
30082317 |
| |
| Submission date |
Feb 23, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Roza Berhanu Lemma |
| E-mail(s) |
[email protected]
|
| Phone |
+4740975997
|
| Organization name |
University of Oslo
|
| Department |
Department of Biosciences
|
| Lab |
Odd Stokke Gabrielsen
|
| Street address |
Blindernveien 31
|
| City |
Oslo |
| ZIP/Postal code |
0371 |
| Country |
Norway |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA435717 |
| SRA |
SRP133372 |
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