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| Status |
Public on Jun 22, 2018 |
| Title |
Expression data from Burkholderia multivorans mucoid and nonmucoid isolates |
| Platform organisms |
Burkholderia cenocepacia J2315; Burkholderia multivorans ATCC 17616 |
| Sample organism |
Burkholderia multivorans |
| Experiment type |
Expression profiling by array
|
| Summary |
Burkholderia multivorans comprises opportunistic bacteria infecting hosts such as cystic fibrosis (CF) patients. Bcc long-term infection of CF patient airways has been associated with emergence of phenotypic variation including the mucoid-to-nonmucoid colony morphotype. Here we studied three Burkholderia multivorans clonal isolates (mucoid D2095 (BM11L), and nonmucoid D2214G and D2214P) and a laboratory nonmucoid variant (BM11L-nmv1) obtained under prolonged stationary phase. The aim is to identify possible traits associated to the different morphotypes and find possible mechanisms for this morphotypic variation.
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| Overall design |
For expression profiling, overnight cultures of the mucoid D2095 and the nonmucoid D2214G, D2214P and D2095R isolates grown in SM medium were diluted to an initial OD640 nm of 0.1 into the same medium. Triplicate samples were cultured at 37ºC with 250 r.p.m. agitation for 17 h and RNA extracted from the two bacterial isolates.
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| Web link |
http://jb.asm.org/content/early/2018/06/12/JB.00216-18
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| Contributor(s) |
Silva IN, Becker JD, Moreira LM |
| Citation(s) |
29914989 |
| |
| Submission date |
Mar 06, 2018 |
| Last update date |
Jun 24, 2018 |
| Contact name |
Leonilde Morais Moreira |
| E-mail(s) |
[email protected]
|
| Phone |
+351 218419031
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| Organization name |
Instituto Superior Tecnico
|
| Department |
Bioengineering
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| Lab |
Biological Sciences
|
| Street address |
A. Rovisco Pais
|
| City |
Lisboa |
| ZIP/Postal code |
1049-001 |
| Country |
Portugal |
| |
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| Platforms (1) |
| GPL13356 |
Affymetrix IST_IBB Burkholderia 15K v1.0 (GeneChip BCC1sa520656F) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA437127 |
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