|
Status |
Public on Apr 11, 2018 |
Title |
Genome-wide analysis of Differential Responses of Pseudomonas aeruginosa PAO1 and PA14 cells to Interfacial Stress |
Organisms |
Pseudomonas aeruginosa PAO1; Pseudomonas aeruginosa PA14 |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Analysis of gene expression level of P. aeruginosa confined to Hexadecane-water interfaces. The hypothesis tested in the present study was to determine if interfacial confinements at hexadecane-water affect microbial metabolism through regulation of stress response.
|
|
|
Overall design |
Total RNA obtained from isolated PAO1 and PA14 cells subjected to 1 hour of confinement at Hexadecane-water interfaces. The Total expression level was compared to the untreated control (exposed for 1 hour to 154 mM NaCl solution only).
|
|
|
Contributor(s) |
Niepa TH |
Citation(s) |
29259206 |
|
Submission date |
Apr 10, 2018 |
Last update date |
Jul 14, 2018 |
Contact name |
Tagbo Niepa |
E-mail(s) |
[email protected]
|
Phone |
4123834265
|
Organization name |
University of Pittsburgh
|
Department |
Chemical and Petroleum Engineering
|
Lab |
MicroBiointerface Lab
|
Street address |
3700 OHara Street, 926 Benedum Hall
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15261 |
Country |
USA |
|
|
Platforms (2) |
GPL18782 |
Illumina HiSeq 2500 (Pseudomonas aeruginosa PAO1) |
GPL21807 |
Illumina HiSeq 2500 (Pseudomonas aeruginosa PA14) |
|
Samples (12)
|
GSM3091026 |
PAO1 Control (exposed to NaCl solution for 1h)_1 |
GSM3091027 |
PAO1 Control (exposed to NaCl solution for 1h)_2 |
GSM3091028 |
PAO1 Control (exposed to NaCl solution for 1h)_3 |
GSM3091029 |
PAO1_FBI (exposed to hexadecane-water interface for 1h)_1 |
GSM3091030 |
PAO1_FBI (exposed to hexadecane-water interface for 1h)_2 |
GSM3091031 |
PAO1_FBI (exposed to hexadecane-water interface for 1h)_3 |
GSM3091032 |
PA14 Control (exposed to NaCl solution for 1h)_1 |
GSM3091033 |
PA14 Control (exposed to NaCl solution for 1h)_2 |
GSM3091034 |
PA14 Control (exposed to NaCl solution for 1h)_3 |
GSM3091035 |
PA14_FBI (exposed to hexadecane-water interface for 1h)_1 |
GSM3091036 |
PA14_FBI (exposed to hexadecane-water interface for 1h)_2 |
GSM3091037 |
PA14_FBI (exposed to hexadecane-water interface for 1h)_3 |
|
Relations |
BioProject |
PRJNA449493 |